HTRA3 抗体 (AA 250-300)
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北京 101111
Quick Overview for HTRA3 抗体 (AA 250-300) (ABIN296848)
抗原
See all HTRA3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 250-300
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特异性
- The amino acid sequence is 100 % homologous in HtrA3, 80 % homologous in HtrA1 and 71 % homologous in HtrA2 and HtrA4.
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预测反应
- Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset (100%) Gorilla, Bovine, Pig, Pufferfish (93%) Mouse, Rat, Panda, Dog, Horse, Turkey, Chicken, Xenopus, Stickleback (87%) Hamster, Elephant, Opossum, Guinea pig, Platypus, Lizard, Salmon, Zebrafish (80%).
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纯化方法
- Protein G purified
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免疫原
- Amino acids 273-287 (IVSTAQREGRELGLR)of human HtrA3 were used as immunogen. Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset (100%), Gorilla, Bovine, Pig, Pufferfish (93%), Mouse, Rat, Panda, Dog, Horse, Turkey, Chicken, Xenopus, Stickleback (87%), Hamster, Elephant, Opossum, Guinea pig, Platypus, Lizard, Salmon, Zebrafish (80%).
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亚型
- IgG
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应用备注
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Approved: IHC, IHC-P (10 μg/mL), WB (1 - 3 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, 0.2 % gelatin, 0.05 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- avoid freeze thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
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- HTRA3 (HtrA Serine Peptidase 3 (HTRA3))
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别名
- HTRA3
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背景
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Name/Gene ID: HTRA3
Subfamily: Serine S1C
Family: Protease
Synonyms: HTRA3, HtrA serine peptidase 3, Serine protease HTRA3, Tasp, Probable serine protease HTRA3, PRSP -
基因ID
- 94031
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UniProt
- P83110
抗原
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