FHL1 抗体
(1 validation)Quick Overview for FHL1 抗体 (ABIN7306298)
抗原
See all FHL1 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes endogenous levels of FHL-1 protein.
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产品特性
- Rabbit polyclonal antibody to FHL-1
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纯化方法
- The antibody was purified by immunogen affinity chromatography.
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免疫原
- Recombinant full length protein of human FHL-1
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anti-Four and A Half LIM Domains 1 (FHL1) (AA 23-120) antibody
FHL1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2A9 unconjugated
anti-Four and A Half LIM Domains 1 (FHL1) (C-Term) antibodyFHL1 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 绵羊, 马, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Four and A Half LIM Domains 1 (FHL1) (Internal Region) antibodyVerified FHL1 适用: 小鼠, 大鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Four and A Half LIM Domains 1 (FHL1) antibodyFHL1 适用: 人, 大鼠, 犬 WB, IF, FACS 宿主: 小鼠 Monoclonal 2E11 unconjugated
anti-Four and A Half LIM Domains 1 (FHL1) (AA 51-100) antibodyFHL1 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 绵羊, 兔, Pig, Hamster WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Four and A Half LIM Domains 1 (FHL1) (AA 214-240), (C-Term) antibodyFHL1 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB19645 unconjugated
anti-Four and A Half LIM Domains 1 (FHL1) (N-Term) antibodyFHL1 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 绵羊, 马, 兔 WB 宿主: 兔 Polyclonal unconjugated
anti-Four and A Half LIM Domains 1 (FHL1) (AA 13-240) antibodyFHL1 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Four and A Half LIM Domains 1 (FHL1) (AA 1-280) antibodyFHL1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Four and A Half LIM Domains 1 (FHL1) (AA 1-280) antibodyFHL1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
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应用备注
- WB (1:500 - 1:2000), IF/IC (1:50 - 1:100)
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限制
- 仅限研究用
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- by
- Alexander Lab, Jacobs School of Medicine and Biomedical Sciences, Department of Medicine, University of Buffalo
- No.
- #101371
- 日期
- 2017.07.19
- 抗原
- FHL-1
- Lot Number
- AL1F04A
- Method validated
- Immunofluorescence
- Positive Control
- MRL/lpr lupus mice (14 weeks of age)
- Negative Control
- FH knockout MRL/lpr lupus mice
- Notes
- Passed. ABIN2966633 works well on murine kidney samples and shows the expected CFH1 localization.
- Primary Antibody
- ABIN2966633
- Secondary Antibody
- goat anti-rabbit rhodamine conjugated antibody (ThermoFisher Scientific, T-2769, lot 6251)
- Full Protocol
- Prepare kidneys of wt and complement factor H ko MRL/lpr lupus mice of 14 weeks of age.
- Snap-freeze kidneys in 2-methylbutane cooled on dry ice and keep at −80°C until use.
- Sections were cut at 8µm using the cryostat.
- Dry sections for 30min at RT.
- Hydrate sections in PBS 2x for 15min at RT.
- Fix cryostat sections with 4% paraformaldehyde (PFA) for 15min at RT.
- Wash sections 2x with PBS for 15min at RT.
- Incubate sections with primary rabbit anti-FHL-1 antibody (antibodies-online, ABIN2966633, AL1F04A) diluted 1:100 in PBS ON at 4°C.
- Wash sections 2x for 15min with PBS.
- Incubate sections with secondary goat anti-rabbit rhodamine conjugated antibody (ThermoFisher Scientific, T-2769, lot 6251) diluted 1:100 in PBS for 30min at RT.
- Wash sections 2x for 15min with PBS.
- Incubate sections with ActinGreen 488 ReadyProbes Reagent (ThermoFisher Scientific, R37122, lot 1700569) diluted 1:100 in PBS for 30min at RT.
- Wash sections 2x for 15min with PBS.
- Image acquisition on a Zeiss microscope (Carl Zeiss, Oberkochen, Germany).
- Experimental Notes
生效 #101371 (Immunofluorescence)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![IF staining with ABIN2966633 of kidneys harvested from 14 week old MRL/lpr mice sufficient and deficient in factor H. Cryosections were fixed, stained and images acquired as described in the protocol.]()
IF staining with ABIN2966633 of kidneys harvested from 14 week old MRL/lpr mice sufficient and deficient in factor H. Cryosections were fixed, stained and images acquired as described in the protocol.
Full Methods -
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状态
- Liquid
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缓冲液
- Liquid in 0.42 % Potassium phosphate, 0.87 % Sodium chloride, pH 7.3, 30 % glycerol, and 0.01 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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有效期
- 12 months
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