IL-33 抗体
Quick Overview for IL-33 抗体 (ABIN296529)
抗原
See all IL-33 (IL33) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes mouse IL-33.
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纯化方法
- Affinity purified
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免疫原
- E. coli-derived rmIL-33.
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亚型
- IgG
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应用备注
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Approved: ELISA (0.5 - 1 μg/mL), Flo (25 μg/mL), IHC, IHC-Fr (2 - 15 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: Suitable for use in Western Blot, Flow Cytometry, ELISA and Immunohistochemistry. Western Blot: 0.1-0.2 μg/mL. Detection limit for rmIL-33 is ~1 ng/lane under non-reducing and reducing conditions. Flow Cytometry: 25 μg/mL Use 10 μL to label 1-5x10^5 cells in a total reaction volume not exceeding 200 μL using activated mouse splenocytes. Immunohistochemistry: 2-15 μg/mL used on frozen mouse intestine samples. Direct ELISA: 0.5-1.0 μg/mL Detection limit for rmIL-33 is ~0.3 ng/well. -
说明
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Target Species of Antibody: Mouse
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Sterile PBS.
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浓度
- Lot specific
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缓冲液
- Lyophilized from PBS, 5 % trehalose.
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- -20 °C
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储存方法
- Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Reconstituted product is stable for 1 year at -20°C.
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- IL-33 (IL33) (Interleukin 33 (IL33))
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别名
- IL33
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背景
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Name/Gene ID: IL33
Family: Interleukin
Synonyms: IL33, C9orf26, DVS27, DVS27-related protein, IL-1F11, IL-33, Interleukin-33, NFEHEV, NF-HEV, Interleukin-1 family member 11, NFHEV, IL1F11, Interleukin 33, RP11-575C20.2 -
基因ID
- 90865
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UniProt
- O95760
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途径
- Production of Molecular Mediator of Immune Response
抗原
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