DPPA5 抗体
Quick Overview for DPPA5 抗体 (ABIN295465)
抗原
See all DPPA5 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Selected for its ability to recognize mouse DPPA5 in the applications listed.
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纯化方法
- Affinity purified
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免疫原
- E. coli-derived rmDPPA5.
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亚型
- IgG
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anti-Developmental Pluripotency Associated 5 (DPPA5) (AA 1-116) antibody
DPPA5 适用: 人 ELISA, WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Developmental Pluripotency Associated 5 (DPPA5) (N-Term) antibodyDPPA5 适用: 人, 小鼠, Pig, Hamster, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Developmental Pluripotency Associated 5 (DPPA5) (AA 2-31), (N-Term) antibodyDPPA5 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB20542 unconjugated
anti-Developmental Pluripotency Associated 5 (DPPA5) (AA 9-39), (N-Term) antibodyDPPA5 适用: 人 WB, FACS, EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: ELISA, ICC, WB
Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse DPPA5. The detection limit for rmDPPA5 is approximately 5 ng/lane under non-reducing and reducing conditions. In this format, this antibody shows approximately 25 % cross-reactivity with rhDPPA5 and approximately 10 % cross-reactivity with rmDPPA4. This antibody has not been optimized for Western blot detection of DPPA5 in cell lysates. Immunocytochemistry: This antibody has been used at a concentration of 10 μg/mL to detect mouse DPPA5 in mouse embryonic stem cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine RedTM-coupled anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse DPPA5. The detection limit for rmDPPA5 is approximately 0.3 ng/well. -
说明
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Target Species of Antibody: Mouse
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- deionized water
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浓度
- Lot specific
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缓冲液
- Lyophilized.
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- DPPA5 (Developmental Pluripotency Associated 5 (DPPA5))
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别名
- DPPA5
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背景
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Name/Gene ID: DPPA5
Synonyms: DPPA5, ESG-1, HDPPA5, ESG1 -
基因ID
- 340168
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UniProt
- A6NC42
抗原
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