HAND2 抗体
Quick Overview for HAND2 抗体 (ABIN294970)
抗原
See all HAND2 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes human HAND2. Species cross-reactivity: Mouse.
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纯化方法
- Affinity purified
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过滤
- 0.2 μm filtered
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免疫原
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E. coli-derived rhHAND2.
Type of Immunogen: Recombinant protein -
亚型
- IgG
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应用备注
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Approved: ELISA (0.5 - 1 μg/mL), IHC (10 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: Suitable for use in ELISA, Western Blot and Immunohistochemistry. ELISA: 0.5-1.0 μg/mL. The detection limit for rhHAND2 and rmHAND2 is approximately 1.3 ng/well. Western Blot: 0.1-0.2 μg/mL. The detection limit for rhHAND2 and rmHAND2 is approximately 25 ng/lane under non-reducing and reducing conditions. Immunohistochemistry: 10 μg/mL to detect HAND2 in mouse embryos. Cells were fixed with PBS containing 4 % paraformaldehyde and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA. After blocking, cells were incubated with diluted primary antibody followed by NorthernLights 557-coupled anti-goat IgG at RT in the dark. Between each step, cells were washed with PBS containing BSA. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute with sterile 40-50 % glycerol, PBS.
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浓度
- Lot specific
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缓冲液
- Lyophilized from a 0.2 um filtered solution in PBS, 5 % trehalose.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
- 4°C or -20°C, Avoid freeze-thaw cycles.
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- HAND2 (Heart and Neural Crest Derivatives Expressed 2 (HAND2))
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别名
- DHAND / HAND2
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背景
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Name/Gene ID: HAND2
Synonyms: HAND2, DHand, DHAND2, Hed, Thing2, BHLHa26 -
基因ID
- 9464
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UniProt
- P61296
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途径
- Tube Formation, Feeding Behaviour
抗原
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