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MBD3 抗体 (AA 215-230)

This 小鼠 单克隆 antibody specifically detects MBD3 in WB, IHC 和 IHC (p). It exhibits reactivity toward 人, 犬, Cow, 马, Pig, Bat, 小鸡 和 非洲爪蟾.
产品编号 ABIN294235
发货至: 中国
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Quick Overview for MBD3 抗体 (AA 215-230) (ABIN294235)

抗原

See all MBD3 抗体
MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))

适用

  • 93
  • 50
  • 37
  • 4
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
人, 犬, Cow, 马, Pig, Bat, 小鸡, 非洲爪蟾

宿主

  • 81
  • 10
  • 2
小鼠

克隆类型

  • 55
  • 38
单克隆

标记

  • 52
  • 6
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
This MBD3 antibody is un-conjugated

应用范围

  • 67
  • 28
  • 25
  • 25
  • 21
  • 15
  • 15
  • 4
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

106B691
  • 抗原表位

    • 10
    • 9
    • 6
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 215-230

    特异性

    The theoretical molecular weight of human MBD2 (411 amino acids) is 45 kD and human MBD3 (291 amino acids) is 33 kD. Based on sequence identity, this will detect human, mouse, and rat MBD3 (100 % ), and human and mouse MBD2 (93 %).

    预测反应

    Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Elephant, Panda, Dog, Bovine, Bat, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus (100%) Marmoset, Mouse, Rat, Hamster, Rabbit, Stickleback, Pufferfish, Zebrafish (94%) Salmon (88%).

    纯化方法

    Protein G purified

    免疫原

    Amino acids 215-230 (CKAFMVTDEDIRKQEE) of human MBD3 protein were used as the immunogen for this antibody. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Elephant, Panda, Dog, Bovine, Bat, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus (100%), Marmoset, Mouse, Rat, Hamster, Rabbit, Stickleback, Pufferfish, Zebrafish (94%), Salmon (88%).

    亚型

    IgG1
  • 应用备注

    Approved: IHC, IHC-P (10 μg/mL), WB (2 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS, 0.02 % sodium azide. Sourced in TCS.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    avoid freeze thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
  • 抗原

    MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))

    别名

    MBD3

    背景

    Name/Gene ID: MBD3

    Synonyms: MBD3

    基因ID

    53615

    UniProt

    O95983

    途径

    Chromatin Binding
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