LPIN3 抗体 (AA 428-529)
This anti-LPIN3 antibody is a 山羊 多克隆 antibody detecting LPIN3 in WB, IHC, ELISA 和 ICC. Suitable for 小鼠.
Quick Overview for LPIN3 抗体 (AA 428-529) (ABIN294094)
抗原
See all LPIN3 抗体
LPIN3
(Lipin 3 (LPIN3))
适用
小鼠
宿主
山羊
克隆类型
多克隆
标记
This LPIN3 antibody is un-conjugated
应用范围
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC)
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抗原表位
- AA 428-529
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特异性
- Selected for its ability to recognize Lipin 3 in the applications listed. In direct ELISAs and Western Blots, this antibody shows ~50 % cross-reactivity with rhLipin 3 and less than 5 % cross-reactivity with rhLipin 1 and rhLipin 2.
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纯化方法
- Affinity purified
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免疫原
- E. coli-derived recombinant mouse Lipin 3 (aa428-529).
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亚型
- IgG
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anti-Lipin 3 (LPIN3) (Internal Region) antibody
Verified LPIN3 适用: 人 IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
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应用备注
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Approved: ELISA (0.5 - 1 μg/mL), ICC (10 μg/mL), IHC (10 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: Suitable for use in ELISA, Western Blot, Immunocytochemistry. Western Blot: 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse rmLipin 3. The detection limit for rmLipin 3 is approximately 2 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 μg/mL to detect Lipin 3 on CCD-18co normal human colon fibroblasts. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody overnight at 4?C followed by donkey anti-goat IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Immunohistochemistry: 10 μg/mL to detect Lipin 3 in cryosections of adult mouse intestine. Tissue was fixed overnight in 4 % PFA + 14 % picric acid in PBS at 4?C. Sections were blocked with 10 % donkey serum and 0.1 % Triton in 1 % BSA in PBS for 45 minutes at RT. After blocking, sections were incubated with primary antibody for 3 hours at RT followed by donkey anti-goat lgG at RT for 1 hour. Between each step, sections were washed with 0.1 % BSA in PBS. Direct ELISA: 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse rmLipin 3. The detection limit for rmLipin 3 is approximately 0.05 ng/well. -
说明
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Target Species of Antibody: Mouse
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Sterile PBS.
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浓度
- Lot specific
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缓冲液
- Lyophilized from PBS, 5 % trehalose
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储存条件
- -20 °C
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储存方法
- Lyophilized powder may be stored at -20°C for short-term only. Reconstituted product is stable for 1 year at -20°C.
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- LPIN3 (Lipin 3 (LPIN3))
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别名
- LPIN3 / LIPIN-3
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背景
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Name/Gene ID: LPIN3
Synonyms: LPIN3, DJ620E11.2, DJ450M14.2, Lipin-3, Lipin 3, Lipin 3-like, Lipin-3-like, SMP2, DJ450M14.3, LIPN3L -
基因ID
- 64900
抗原
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