CDH6 抗体 (Extracellular Domain)
Quick Overview for CDH6 抗体 (Extracellular Domain) (ABIN293426)
抗原
See all CDH6 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Extracellular Domain
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特异性
- Recognizes human Cadherin-6. In direct ELISAs and Western Blot, shows less than 5 % cross-reactivity with rhCadherin-8, rhCadherin-11, and rhCadherin-12.
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纯化方法
- Immunoaffinity purified
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免疫原
- NS0-derived rhCadherin-6 extracellular domain.
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亚型
- IgG
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anti-K-Cadherin (CDH6) (AA 281-380) antibody
CDH6 适用: 人 WB, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-K-Cadherin (CDH6) antibodyCDH6 适用: 人, 大鼠 WB, IHC, IHC (fro), ICC 宿主: 小鼠 Monoclonal 2B6 unconjugated
anti-K-Cadherin (CDH6) (AA 513-612) antibodyCDH6 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 2F2 unconjugated
anti-K-Cadherin (CDH6) antibodyCDH6 适用: 人 WB, IP 宿主: 兔 Monoclonal unconjugated
anti-K-Cadherin (CDH6) (AA 1-250) antibodyCDH6 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-K-Cadherin (CDH6) antibodyCDH6 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-K-Cadherin (CDH6) (AA 665-695), (C-Term) antibodyCDH6 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-K-Cadherin (CDH6) (AA 1-663) antibodyCDH6 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-K-Cadherin (CDH6) (C-Term) antibodyCDH6 适用: 人, 大鼠, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-K-Cadherin (CDH6) antibodyCDH6 适用: 人, 大鼠, 小鼠, Cow, 小鸡 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: ELISA (0.5 - 1 μg/mL), Flo (50 μg/mL), ICC (10 μg/mL), WB
Usage: Suitable for use in Western Blot, Immunocytochemistry, Flow Cytometry and ELISA. Western Blot: 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human Cadherin-6. The detection limit is approximately 10 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 μg/mL to detect human Cadherin-6 in MG63 human osteosarcoma cells cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody for 3 hours at RT followed by donkey anti-sheep lgG at RT for 1 hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Flow Cytometry: 50 μg/mL Add 10 μL of the diluted solution to 1-2.5x10^5 MG63 human osteosarcoma cells. This antibody has been tested on MG63 human osteosarcoma cells. Dilute this antibody to 50 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200uL. The binding of unlabeled antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as anti-sheep lgG conjugated to a fluorochrome. Direct ELISA: 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human Cadherin-6. The detection limit for rhCadherin-6 is approximately 1 ng/well. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Sterile PBS.
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浓度
- Lot specific
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缓冲液
- Lyophilized from PBS, 5 % trehalose.
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储存条件
- -20 °C
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储存方法
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Short term: -20°C
Long term: -20°C.
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- CDH6 (K-Cadherin (CDH6))
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别名
- CDH6 / K Cadherin
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背景
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Name/Gene ID: CDH6
Family: Cadherin
Synonyms: CDH6, K Cadherin, K-cadherin, CAD6, Cadherin-6, Fetal kidney cadherin, KCAD, Kidney cadherin -
基因ID
- 1004
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UniProt
- P55285
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途径
- Cell-Cell Junction Organization
抗原
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