UTF1 抗体 (AA 44-153)
Quick Overview for UTF1 抗体 (AA 44-153) (ABIN293228)
抗原
See all UTF1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 44-153
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特异性
- Selected for its ability to recognize UTF1 in the applications listed.
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纯化方法
- Affinity purified
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过滤
- 0.2 μm filtered
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免疫原
- E. coli-derived rhUTF1 (aa44-153).
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亚型
- IgG
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anti-Undifferentiated Embryonic Cell Transcription Factor 1 (UTF1) (AA 148-214) antibody
UTF1 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 5B6 unconjugated
anti-Undifferentiated Embryonic Cell Transcription Factor 1 (UTF1) (AA 325-359), (C-Term) antibodyUTF1 适用: 小鼠 WB 宿主: 兔 Polyclonal RB52502 unconjugated
anti-Undifferentiated Embryonic Cell Transcription Factor 1 (UTF1) (AA 148-214) antibodyUTF1 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal unconjugated
anti-Undifferentiated Embryonic Cell Transcription Factor 1 (UTF1) (AA 164-199) antibodyUTF1 适用: 人 WB 宿主: 兔 Polyclonal RB51421 unconjugated
anti-Undifferentiated Embryonic Cell Transcription Factor 1 (UTF1) (AA 251-300) antibodyUTF1 适用: 人, 小鼠, 大鼠, 豚鼠 WB, IHC (p), IHC 宿主: 兔 Polyclonal unconjugated
anti-Undifferentiated Embryonic Cell Transcription Factor 1 (UTF1) (AA 191-224) antibodyUTF1 适用: 人 WB 宿主: 兔 Polyclonal RB51420 unconjugated
anti-Undifferentiated Embryonic Cell Transcription Factor 1 (UTF1) (Internal Region) antibodyVerified UTF1 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Undifferentiated Embryonic Cell Transcription Factor 1 (UTF1) (N-Term) antibodyUTF1 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Undifferentiated Embryonic Cell Transcription Factor 1 (UTF1) (Middle Region) antibodyUTF1 适用: 人, 小鼠, 大鼠, 豚鼠, Cow, 马, Pig WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: ELISA, ICC, WB
Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect UTF1. The detection limit for rhUTF1 is approximately 10 ng/lane and 1 ng/lane under non-reducing and reducing conditions, respectively. Immunocytochemistry: This antibody can be used at a concentration of 10 μg/mL to detect UTF1 in mouse D3 cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Northern Lights 557-coupled anti-donkey IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect UTF1. The detection limit for rhUTF1 is approximately 1.0 ng/well. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Sterile 40-50 % glycerol, PBS
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浓度
- Lot specific
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缓冲液
- Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5 % trehalose.
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注意事项
- Aviod frequent freeze thaws.
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储存条件
- -20 °C
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储存方法
- Store at -20°C prior to reconstitution. After reconstitution aliquot and freeze at-20°C. Avoid freeze-thaw cycles.
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- UTF1 (Undifferentiated Embryonic Cell Transcription Factor 1 (UTF1))
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别名
- UTF1
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背景
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Name/Gene ID: UTF1
Synonyms: UTF1 -
基因ID
- 8433
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UniProt
- Q5T230
抗原
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