alpha Tubulin 抗体 (AA 426-430, AA 426-450)
Quick Overview for alpha Tubulin 抗体 (AA 426-430, AA 426-450) (ABIN293217)
抗原
See all alpha Tubulin (TUBA1) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 426-430, AA 426-450
-
特异性
- Recognizes chicken tubulin alpha (MW 57kD). Causes the 10nm filaments to collapse into large lateral aggregates collecting in the cell periphery or tight juxtanuclear caps. Does not block microtubule assembly. Does not inhibit polymerization or depolymerization of platelet tubulin in vitro. Blocks (70-80 % ) the ability of tubulin dimers (with GppNHp bound) to promote a stable inhibition of adenylyl cyclase. Species cross-reactivity: bovine, human, porcine, rat, mouse, guinea pig, gerbil and frog.
-
纯化方法
- Protein G purified
-
免疫原
-
Native chick brain microtubules. Epitope: Localizes between aa 426-450. aa 426-430 are critical for reactivity (2). Cellular Localization: Cytoplasmic.
Type of Immunogen: Purified protein -
亚型
- IgG1 kappa
-
-
-
-
应用备注
-
Approved: IF, IHC, IHC-Fr, IHC-P, IP, WB (1 - 2 μg/mL)
Usage: Suitable for use in Electron Microscopy, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Western Blot: 1-2 μg/mL for 2hrs at RT. Immunohistochemistry (Frozen and formalin/paraffin): 1:200 for 20 min at RT. Immunoprecipitation (Native and denatured): 2 μg/mg protein lysate using Protein G. Positive control: LS174T, MAD109 cells. Skin or lung. -
说明
-
Target Species of Antibody: Chicken
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- Lot specific
-
缓冲液
- PBS, pH 7.4, 0.2 % BSA, 15 mM sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
-
- alpha Tubulin (TUBA1)
-
别名
- Alpha Tubulin
-
途径
- Microtubule Dynamics
抗原
-