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alpha Tubulin 抗体 (AA 426-430, AA 426-450)

This anti-alpha Tubulin antibody is a 小鼠 单克隆 antibody detecting alpha Tubulin in WB, IHC, IF, IHC (p), IP 和 IHC (fro). Suitable for 人, 小鼠, 大鼠, Pig, Cow, 小鸡, 豚鼠 和 Frog.
产品编号 ABIN293217
发货至: 中国

Quick Overview for alpha Tubulin 抗体 (AA 426-430, AA 426-450) (ABIN293217)

抗原

See all alpha Tubulin (TUBA1) 抗体
alpha Tubulin (TUBA1)

适用

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人, 小鼠, 大鼠, Pig, Cow, 小鸡, 豚鼠, Frog

宿主

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小鼠

克隆类型

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单克隆

标记

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This alpha Tubulin antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • 抗原表位

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    AA 426-430, AA 426-450

    特异性

    Recognizes chicken tubulin alpha (MW 57kD). Causes the 10nm filaments to collapse into large lateral aggregates collecting in the cell periphery or tight juxtanuclear caps. Does not block microtubule assembly. Does not inhibit polymerization or depolymerization of platelet tubulin in vitro. Blocks (70-80 % ) the ability of tubulin dimers (with GppNHp bound) to promote a stable inhibition of adenylyl cyclase. Species cross-reactivity: bovine, human, porcine, rat, mouse, guinea pig, gerbil and frog.

    纯化方法

    Protein G purified

    免疫原

    Native chick brain microtubules. Epitope: Localizes between aa 426-450. aa 426-430 are critical for reactivity (2). Cellular Localization: Cytoplasmic.

    Type of Immunogen: Purified protein

    亚型

    IgG1 kappa
  • 应用备注

    Approved: IF, IHC, IHC-Fr, IHC-P, IP, WB (1 - 2 μg/mL)

    Usage: Suitable for use in Electron Microscopy, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Western Blot: 1-2 μg/mL for 2hrs at RT. Immunohistochemistry (Frozen and formalin/paraffin): 1:200 for 20 min at RT. Immunoprecipitation (Native and denatured): 2 μg/mg protein lysate using Protein G. Positive control: LS174T, MAD109 cells. Skin or lung.

    说明

    Target Species of Antibody: Chicken

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS,  pH 7.4, 0.2 % BSA, 15 mM sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • 抗原

    alpha Tubulin (TUBA1)

    别名

    Alpha Tubulin

    途径

    Microtubule Dynamics
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