Versican 抗体 (AA 1344-1554)
Quick Overview for Versican 抗体 (AA 1344-1554) (ABIN292878)
抗原
See all Versican (Vcan) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1344-1554
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特异性
- Recognizes human Versican. Species sequence Homology: For immunizing sequence, aa1344-1554: canine (80 % ), mouse (60 %).
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纯化方法
- Immunoaffinity purified
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免疫原
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Recombinant protein corresponding to aa1344-1554 from human Versican isoform V0, expressed in E. coli (P13611).
Type of Immunogen: Recombinant protein -
亚型
- IgG
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anti-Versican (Vcan) (AA 3301-3396) antibody
Vcan 适用: 小鼠, 大鼠 ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Versican (Vcan) (AA 3058-3299) antibodyVcan 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Versican (Vcan) antibodyVcan 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Versican (Vcan) (AA 2958-3209) antibodyVcan 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Versican (Vcan) (AA 362-585) antibodyVcan 适用: 小鼠 WB, ICC, IF 宿主: 小鼠 Monoclonal S351-23 unconjugated
anti-Versican (Vcan) (AA 362-585) antibody (HRP)Vcan 适用: 小鼠 WB, ICC, IF 宿主: 小鼠 Monoclonal S351-23 HRP
anti-Versican (Vcan) (AA 362-585) antibody (FITC)Vcan 适用: 小鼠 WB, ICC, IF 宿主: 小鼠 Monoclonal S351-23 FITC
anti-Versican (Vcan) (AA 362-585) antibody (Biotin)Vcan 适用: 小鼠 WB, ICC, IF 宿主: 小鼠 Monoclonal S351-23 Biotin
anti-Versican (Vcan) (AA 362-585) antibody (PE)Vcan 适用: 小鼠 WB, ICC, IF 宿主: 小鼠 Monoclonal S351-23 PE
anti-Versican (Vcan) (AA 362-585) antibody (APC)Vcan 适用: 小鼠 WB, ICC, IF 宿主: 小鼠 Monoclonal S351-23 APC
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应用备注
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Approved: ELISA (0.5 - 1 μg/mL), ICC (10 μg/mL), IHC (10 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: Suitable for use in ELISA, Western Blot, Immunocytochemistry and Immunohistochemistry. Western Blot: 0.1-0.2 μg/mL. Detection limit for rhVersican is ~2 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 μg/mL detects Versican in Hela cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by fluorochrome-coupled anti-goat IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Immunohistochemistry: 10 μg/mL detects Versican in cryosections of E13.5 mouse embryos. Tissue was fixed with PBS containing 4 %PFA for 20 min. at RT and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton and 1 % BSA for 45 minutes at RT. After blocking, sections were incubated with diluted primary antibody for 3 hours at RT followed by fluorochrome-coupled anti-goat lgG at RT for 1 hour. Between each step, sections were washed with 0.1 % BSA in PBS. Direct ELISA: 0.5-1.0 μg/mL. Detection limit for rhVersican is ~0.1 ng/well. Optimal dilutions should be determined by each laboratory. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Sterile PBS
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浓度
- Lot specific
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缓冲液
- Lyophilized from PBS, 5 % trehalose.
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注意事项
- Avoid frequent freeze thaws. Store undiluted.
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储存条件
- -20 °C,-80 °C
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储存方法
- Store at -20°C to -70°C. Stable for 1 year from date of receipt.
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- Versican (Vcan)
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别名
- VCAN / Versican
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背景
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Name/Gene ID: VCAN
Synonyms: VCAN, CSPG2, ERVR, GHAP, Large fibroblast proteoglycan, PG-M, Versican, WGN, Versican core protein, Versican proteoglycan, WGN1 -
基因ID
- 1462
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UniProt
- P13611
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途径
- Glycosaminoglycan Metabolic Process
抗原
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