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HAVCR1 抗体 (AA 200-300)

This 兔 多克隆 antibody specifically detects HAVCR1 in WB, IHC 和 IHC (p). It exhibits reactivity toward 人 和 小鼠.
产品编号 ABIN292372
发货至: 中国
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Quick Overview for HAVCR1 抗体 (AA 200-300) (ABIN292372)

抗原

See all HAVCR1 抗体
HAVCR1 (Hepatitis A Virus Cellular Receptor 1 (HAVCR1))

适用

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人, 小鼠

宿主

  • 111
  • 28
  • 8
  • 4
  • 2

克隆类型

  • 108
  • 41
  • 3
多克隆

标记

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This HAVCR1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 抗原表位

    • 8
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    AA 200-300

    特异性

    The amino acid sequence (SPLYSYTTDGNDTVT) used as immunogen for this is 100 % homologous in human, 93 % homologous in vervet/green monkey and 80 % homologous in chimp. Isoforms of different molecular weights have been described for TIM-1. The sequence used for immunogen is conserved between TIM-1 (364aa, NP_036338) and TIM-1 isoform CRA_a (194aa, EAW61615.1). Therefore, the molecular weight observed in western blots may vary depending on the forms present.

    预测反应

    Percent identity by BLAST analysis: Human, Gorilla (100%) Monkey (93%) Dog (80%).

    纯化方法

    Protein G purified

    免疫原

    A synthetic peptide (SPLYSYTTDGNDTVT) of human HAVCR1/TIM-1protein were used as immunogen. This sequence corresponds to amino acids (aa) 248-262 of NP_036338 and aa 84-98 of EAW61615.1. Isoforms of HAVCR1/TIM-1 have been described. For example, NP_036338 is 364 aa and EAW61615.1 is 194 aa. EAW61615.1 is referred to as isoform CRA_a. Percent identity by BLAST analysis: Human, Gorilla (100%), Monkey (93%), Dog (80%).

    Type of Immunogen: Synthetic peptide

    亚型

    IgG
  • 应用备注

    Approved: IHC, IHC-P (2.5 - 5 μg/mL), WB (1 - 3 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5-5 μg/mL.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS, 0.05 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at 4°C for short term applications. For long term storage, aliquot and store at -20°C.
  • 抗原

    HAVCR1 (Hepatitis A Virus Cellular Receptor 1 (HAVCR1))

    别名

    HAVCR1 / KIM-1

    物质类

    Virus

    背景

    Name/Gene ID: HAVCR1

    Synonyms: HAVCR1, HAVCR-1, KIM-1, Kidney injury molecule 1, KIM1, T-cell membrane protein 1, TIM1, TIMD1, TIM-1, HAVCR, TIMD-1

    基因ID

    26762

    NCBI登录号

    NP_036338

    UniProt

    Q96D42
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