DPPA4 抗体 (AA 125-296)
Quick Overview for DPPA4 抗体 (AA 125-296) (ABIN292183)
抗原
See all DPPA4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 125-296
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特异性
- Selected for its ability to recognize mouse DPPA4 in the applications listed. In direct ELISAs and Western Blots, this antibody shows less than 5 % cross-reactivity with rhDPPA4 and rmDPPA5.
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纯化方法
- Affinity purified
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免疫原
- E. coli-derived rmDPPA4 (aa125-296).
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亚型
- IgG
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anti-Developmental Pluripotency Associated 4 (DPPA4) (AA 1-304) antibody
DPPA4 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Developmental Pluripotency Associated 4 (DPPA4) (AA 11-264) antibodyDPPA4 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Developmental Pluripotency Associated 4 (DPPA4) (AA 1-50) antibodyDPPA4 适用: 人, 小鼠 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Developmental Pluripotency Associated 4 (DPPA4) (AA 9-254) antibodyDPPA4 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal 1E1 unconjugated
anti-Developmental Pluripotency Associated 4 (DPPA4) (AA 50-79), (N-Term) antibodyDPPA4 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Developmental Pluripotency Associated 4 (DPPA4) (AA 107-142) antibodyDPPA4 适用: 人 WB 宿主: 兔 Polyclonal RB51241 unconjugated
anti-Developmental Pluripotency Associated 4 (DPPA4) (AA 1-294) antibodyDPPA4 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Developmental Pluripotency Associated 4 (DPPA4) (AA 1-294) antibodyDPPA4 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Developmental Pluripotency Associated 4 (DPPA4) (AA 15-304) antibodyDPPA4 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Developmental Pluripotency Associated 4 (DPPA4) (N-Term) antibodyDPPA4 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: ELISA, ICC, WB
Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse DPPA4. The detection limit for rmDPPA4 is approximately 1 ng/lane under non-reducing and reducing conditions. This antibody has not been optimized for Western blot detection of DPPA4 in cell lysates. Immunocytochemistry: This antibody has been used at a concentration of 10 μg/mL to detect mouse DPPA4 in mouse embryonic stem cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine RedTM-coupled anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse DPPA4. The detection limit for rmDPPA4 is approximately 0.2 ng/well. -
说明
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Target Species of Antibody: Mouse
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- deionized water
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浓度
- Lot specific
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缓冲液
- Lyophilized.
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- DPPA4 (Developmental Pluripotency Associated 4 (DPPA4))
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别名
- DPPA4
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背景
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Name/Gene ID: DPPA4
Synonyms: DPPA4 -
基因ID
- 55211
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UniProt
- Q7L190
抗原
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