Integrin beta 5 抗体 (Extracellular Domain)
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Quick Overview for Integrin beta 5 抗体 (Extracellular Domain) (ABIN291993)
抗原
See all Integrin beta 5 (ITGB5) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Extracellular Domain
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特异性
- Recognizes human Integrin b5 extracellular domain. In Direct ELISAs and Western Blots, this antibody shows less than 5 % cross-reactivity with rhIntegrin b1, rhIntegrin b3, rhIntegrin b4, rhIntegrin b6, and rmIntegrin b7. Species sequence Homology: mouse, 91 %, canine, 89 %.
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纯化方法
- Affinity purified
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免疫原
- CHO cell-derived rhIntegrin b5 extracellular domain.
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亚型
- IgG
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anti-Integrin beta 5 (ITGB5) (AA 421-516) antibody
ITGB5 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 2C4 unconjugated
anti-Integrin beta 5 (ITGB5) (AA 137-378) antibodyITGB5 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Integrin beta 5 (ITGB5) (AA 136-378) antibodyITGB5 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Integrin beta 5 (ITGB5) (C-Term) antibodyITGB5 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Integrin beta 5 (ITGB5) (C-Term) antibodyITGB5 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Integrin beta 5 (ITGB5) (AA 421-516) antibodyITGB5 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Integrin beta 5 (ITGB5) (C-Term) antibodyITGB5 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Integrin beta 5 (ITGB5) (AA 690-770) antibodyITGB5 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Integrin beta 5 (ITGB5) (AA 1-799) antibodyITGB5 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Integrin beta 5 (ITGB5) (AA 216-244), (N-Term) antibodyITGB5 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB33832 unconjugated
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应用备注
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Approved: ELISA (0.5 - 1 μg/mL), Flo, ICC (10 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: Suitable for use in Western Blot, Flow Cytometry, Immunocytochemistry and ELISA. Western Blot: 0.1-0.2 μg/mL. Detection limit ~5 ng/lane under non-reducing and reducing conditions. Flow Cytometry: Dilute antibody to 50 μg/mL and add 10 μL of the diluted solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as anti-sheep lgG conjugated to a fluorochrome. Immunocytochemistry: 10 μg/mL to detect b5 in MG-63 human osteosarcoma cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine Red coupled anti-sheep IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA: 0.5-1.0 μg/mL. Detection limit ~0.2 ng/well. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Sterile PBS
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浓度
- Lot specific
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缓冲液
- Lyophilized, PBS, 5 % trehalose
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
- 4°C or -20°C, Avoid freeze-thaw cycles.
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- Integrin beta 5 (ITGB5)
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别名
- ITGB5 / Integrin Beta 5
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背景
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Name/Gene ID: ITGB5
Family: Integrin
Synonyms: ITGB5, Integrin Beta 5, Integrin beta-5, Integrin beta-5 subunit, Integrin, beta 5 -
基因ID
- 3693
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UniProt
- P18084
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途径
- Integrin Complex
抗原
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