HES4 抗体 (AA 94-221)
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北京 101111
Quick Overview for HES4 抗体 (AA 94-221) (ABIN291868)
抗原
See all HES4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 94-221
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特异性
- Selected for its ability to recognize human HES-4 in the applications listed below. In direct ELISAs and Western Blots, this antibody shows less than 1 % cross-reactivity with rhHES-1, rhHES-2, rhHES-5, and rhHES-7.
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纯化方法
- Affinity purified
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免疫原
- E. coli-derived rhHES-4 (aa94-221).
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亚型
- IgG
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anti-Hairy and Enhancer of Split 4 (HES4) (Internal Region) antibody
Verified HES4 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Hairy and Enhancer of Split 4 (HES4) (AA 1-221) antibodyHES4 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Hairy and Enhancer of Split 4 (HES4) (AA 141-190) antibodyHES4 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Hairy and Enhancer of Split 4 (HES4) (N-Term) antibodyHES4 适用: 人, Cow, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Hairy and Enhancer of Split 4 (HES4) (N-Term) antibodyHES4 适用: 人, Cow, 犬, Pig, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: ELISA, ICC, WB
Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human HES-4. The detection limit for rhHES-4 is approximately 5 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: This antibody has been used at a concentration of 10 μg/mL to detect HES-4 in HEK293 cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by fluorochrome-coupled anti-sheep IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human HES-4. The detection limit for rhHES-4 is approximately 0.2 ng/well. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- deionized water
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浓度
- Lot specific
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缓冲液
- Lyophilized.
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- HES4 (Hairy and Enhancer of Split 4 (HES4))
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别名
- HES4
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背景
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Name/Gene ID: HES4
Synonyms: HES4, BHLHb42, Transcription factor HES-4, BHLH factor Hes4, Hairy and enhancer of split 4, HHES4 -
基因ID
- 57801
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UniProt
- Q9HCC6
抗原
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