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HSP70 抗体

This anti-HSP70 antibody is a 小鼠 单克隆 antibody detecting HSP70 in WB, IHC, IF, IP, FACS, IHC (p) 和 IHC (fro). Suitable for 人, Cow 和 猴.
产品编号 ABIN291550
发货至: 中国

Quick Overview for HSP70 抗体 (ABIN291550)

抗原

See all HSP70 抗体
HSP70 (Heat Shock Protein 70 (HSP70))

适用

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人, Cow, 猴

宿主

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小鼠

克隆类型

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单克隆

标记

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This HSP70 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • 特异性

    Recognizes both the constitutive (HSP73) and inducible (HSP72) forms of HSP70. Species cross-reactivity: Human, monkey and bovine.

    纯化方法

    Protein A purified

    免疫原

    HSP70 protein from HeLa cells. Cellular Localization: Nuclear and cytoplasmic.

    亚型

    IgG2a
  • 应用备注

    Approved: Flo, IF, IHC, IHC-Fr, IHC-P, IP, WB (0.5 - 1 μg/mL)

    Usage: Suitable for use in Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Immunoprecipitation (Native & denatured): 2 μg antibody/mg protein lysate using Protein A. Western Blot: 0.5-1 μg/mL for 2hrs at RT. Immunohistochemistry (Frozen & formalin/paraffin): 2-4 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0 for 10-20 min., followed by cooling at RT for 20 min. Positive control: BT474 or HeLa cells, breast carcinoma. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS,  pH 7.4.

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    Short term: 4°C
    Long term: Add glycerol (40-50%) -20°C.
  • 抗原

    HSP70 (Heat Shock Protein 70 (HSP70))

    别名

    HSP70 / Heat Shock Protein 70

    背景

    Name/Gene ID: HSP70

    Synonyms: HSP70
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