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Keratin 10/13 抗体

The 小鼠 单克隆 anti-Keratin 10/13 antibody (Clone DE-K13) (ABIN291339) specifically detects Keratin 10/13 in WB, IHC, IHC (p) 和 IHC (fro). The antibody is reactive with 人 samples.
产品编号 ABIN291339
发货至: 中国
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Quick Overview for Keratin 10/13 抗体 (ABIN291339)

抗原

Keratin 10/13

适用

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宿主

  • 15
小鼠

克隆类型

  • 15
单克隆

标记

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非结合性

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

克隆位点

DE-K13
  • 特异性

    Reacts with Cytokeratin 10 (56.5 kD) and Cytokeratin 13 (53 kD). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues. DE-K13 recognizes only Cytokeratin 13 on formalin-fixed, paraffin-embedded tissue sections.

    纯化方法

    Protein A purified

    免疫原

    Cytoskeletal preparation extracted from human ectocervical epithelium.

    Type of Immunogen: Protein fraction

    亚型

    IgG2a
  • 应用备注

    Approved: IHC, IHC-Fr, IHC-P, WB (1:100, 1 - 3 μg/mL)

    Usage: Western Blotting: Recommended dilution - 1-3 μg/mL. Immunohistochemistry (paraffin sections): Staining technique: Standard ABC technique (DAB+). Recommended dilution - 10 μg/mL (1:100). Positive tissue - tonsil. Pretreatment: 0.1 % pepsin in 0.1 M HCl for

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS, 15 mM sodium azide, approx,  pH 7.4.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    avoid freeze thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
  • 抗原

    Keratin 10/13

    别名

    Cytokeratin 10+13

    背景

    This antibody detects: Cytokeratin 10+13
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