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SOD1 抗体 (AA 2-154)

This anti-SOD1 antibody is a 兔 多克隆 antibody detecting SOD1 in WB, IHC, IP 和 IF/ICC. Suitable for 人 和 马. Independently validated for use in Western Blotting, Immunocytochemistry. This Primary Antibody has been cited in 1 publication.
Pubmed (1 reference) Independent Validation (2 validations)
产品编号 ABIN2913314
发货至: 中国

Quick Overview for SOD1 抗体 (AA 2-154) (ABIN2913314)

抗原

See all SOD1 抗体
SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

适用

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人, 马

宿主

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兔

克隆类型

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多克隆

标记

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This SOD1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (fixed cells) (IF/ICC)
Discover our top product SOD1 Primary Antibody

  • 抗原表位

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    AA 2-154

    纯化方法

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    免疫原

    SOD1 (Ala2-Pro154)

    亚型

    IgG
  • anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 1-154) antibody
    anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 1-154) antibody

    SOD1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

    anti-Superoxide Dismutase 1, Soluble (SOD1) antibody
    anti-Superoxide Dismutase 1, Soluble (SOD1) antibody

    KO Validated SOD1 适用: 人 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated

    anti-Superoxide Dismutase 1, Soluble (SOD1) (C-Term) antibody
    anti-Superoxide Dismutase 1, Soluble (SOD1) (C-Term) antibody

    KO Validated SOD1 适用: 人 WB, IHC (fro) 宿主: 兔 Polyclonal unconjugated

    anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 6-100) antibody
    anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 6-100) antibody

    SOD1 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, IHC (p), FACS, IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated

    anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 2-154) antibody
    anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 2-154) antibody

    SOD1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

    anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 2-154) antibody
    anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 2-154) antibody

    SOD1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

    anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 2-153) antibody (APC)
    anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 2-153) antibody (APC)

    SOD1 适用: 兔 WB, IHC 宿主: 豚鼠 Polyclonal APC

    anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 2-154) antibody
    anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 2-154) antibody

    SOD1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

    anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 2-153) antibody
    anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 2-153) antibody

    SOD1 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

    anti-Superoxide Dismutase 1, Soluble (SOD1) antibody
    anti-Superoxide Dismutase 1, Soluble (SOD1) antibody

    SOD1 适用: 人, 小鼠 WB, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 6F5 unconjugated

  • 应用备注

    Optimal working dilution should be determined by the investigator.

    限制

    仅限研究用
  • 生效 #100074 (Western Blotting)
    '独立验证'标志
    by
    Royal Veterinary College, London
    No.
    #100074
    日期
    2016.11.17
    抗原
    SOD-1
    Lot Number
    Method validated
    Western Blotting
    Positive Control
    Horse Fibroblasts, human SH-SY5Y
    Negative Control
    Notes
    ABIN3058918 successfully labels SOD-1 in lysates of equine fibroblast and human SH-SY5Y cells.
    '独立验证'标志
    Validation Images
    Total protein lysates from horse fibroblasts and human SH-SY5Y cells were separated were separated on a polyacrylamide gel under denaturing conditions. SOD-1 and TUBB protein bands were revealed as described in the protocol section. Total protein lysates from horse fibroblasts and human SH-SY5Y cells were separated were separated on a polyacrylamide gel under denaturing conditions. SOD-1 and TUBB protein bands were revealed as described in the protocol section.
    Full Methods
    Primary Antibody
    ABIN3058918
    Secondary Antibody
    Goat anti-rabbit-IgG HRP-linked whole antibody (Dako, D0487)
    Full Protocol
    • Seed 2x105 horse fibroblasts in a T25 flask and grow for 48h at 5% CO2 in supplemented with Dulbecco’s modified Eagle media (Sigma-Aldrich, D5546), with 10% heat-inactivated fetal calf serum (FCS) and 2mM (1%) L-glutamine and 1% penicillin (10IU/ml)/streptomycin (100µg/ml) to form a monolayer of 70-90%.
    • Human SH-SY5Y are cultured in identical conditions.
    • Wash cells once ice cold PBS.
    • Add 500µl chilled RIPA buffer (50mM Tris pH 7.5, 150mM NaCl, 1% NP40, 0.5% Na-deoxychlorate, 0.5% SDS, 1mM EDTA, 1mM EGTA, 1mM PMSF and 1x protease inhibitor) to each flask and incubate for 1min on ice.
    • Scrape each monolayer and transfer protein aggregates to a separate 1.5ml microcentrifuge tube.
    • Incubate for 20min on ice.
    • Centrifuge at 14000rpm for 20min at 4°C.
    • Remove the supernatant and determine the concentration of soluble proteins against a BSA protein standard using a DC protein assay (Biorad, 5000112).
    • Adjust protein samples to 0.3mg/ml total protein, using RIPA buffer and loading buffer.
    • After boiling for 5-10min, 1-10µg of each sample is separated on an Any kD Mini-PROTEAN TGX Stain-Free Precast Gel (Biorad, 4568123) at 100V for 90min.
    • Transfer proteins onto a PVDF blotting membrane (Hybond P, GE Healthcare Life Sciences, RPN303D) for 90min at 300mA.
    • Block the membrane with PBST containing 10% dry milk for 1h at RT.
    • Incubation with primary SOD-1 antibody (antibodies-online, ABIN3058918) diluted 1:1000 and a TUBB loading control antibody (abcam, ab6046) diluted 1:1000 in PBST containing 5% dry milk ON at 4°C.
    • Wash 5 times with PBST over 1 hour whilst shaking.
    • Incubation with secondary goat anti-rabbit-IgG horseradish peroxidase-linked whole antibody (Dako, D0487) diluted 1:5000 in PBST for 1h at RT.
    • Wash 5 times with PBST over 1 hour whilst shaking.
    • Blots were developed with Amersham ECL Prime Western Blotting Detection reagent (GE Healthcare Life Sciences, RPN2232) and visualized on a Kodak developer.
    Experimental Notes
    The same human cell lysates were also used for a different blot with an anti-human SOD-1 antibody (lab stock). A band of the same molecular weight as in the horse fibroblast lysates was revealed.
  • 生效 #100075 (Immunocytochemistry)
    '独立验证'标志
    by
    Royal Veterinary College, London
    No.
    #100075
    日期
    2016.11.17
    抗原
    SOD-1
    Lot Number
    Method validated
    Immunocytochemistry
    Positive Control
    Horse Fibroblasts
    Negative Control
    Notes
    ABIN3058918 achieves SOD-1 staining in horse fibroblasts when grown in a monolayer.
    '独立验证'标志
    Validation Images
    Horse fibroblasts were grown in a monolayer and stained with ABIN3058918 (red) as desecribed in the protocol. DAPI (blue) was used to visualize nuclei. Horse fibroblasts were grown in a monolayer and stained with ABIN3058918 (red) as desecribed in the protocol. DAPI (blue) was used to visualize nuclei.
    Full Methods
    Primary Antibody
    ABIN3058918
    Secondary Antibody
    anti-rabbit labeled with Alexa Fluor 594
    Full Protocol
    • Horse fibroblasts are seeded onto glass coverslips pre-treated with 1:10 diluted Matrigel (BD Biosciences, 354234) at a density of 1x105 cells per coverslip in a 24 well plate.
    • Incubate for 48h at 37°C in 5% CO2.
    • Wash coverslips with PBS.
    • Fix coverslips with 4% paraformaldehyde (PFA) and 0.1% triton for 20min at 4°C, followed by 8% PFA for 30min at RT.
    • Permeabilize cells with 0.5% triton for 30min at RT.
    • Wash coverslips 3 times with PBS.
    • Incubate coverslips with primary rabbit anti SOD-1 antibody (antibodies-online, ABIN3058918) diluted 1:40 in a dark, humidified chamber for 1h at RT. A negative control was incubated without primary antibody.
    • Wash coverslips 3 times with PBS.
    • Incubate with secondary goat anti rabbit labeled with Alexa Fluor 594 (ThermoFisher Scientific, A10239) diluted 1:500 for 1h at RT.
    • Wash coverslips 3 times with PBS.
    • Mount coverslips in a hard setting mount with VECTASHIELD HardSet Antifade Mounting Medium with DAPI (Vector Laboratories, H-1500).
    • Fluorescence microscopy is then used to image each coverslip.
    Experimental Notes

  • 状态

    Liquid

    浓度

    500 μg/mL

    缓冲液

    0.01M PBS,  pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

    储存液

    ProClin

    注意事项

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at 4 °C for frequent use. Aliquot and store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
  • Kronstein-Wiedemann, Stadtmüller, Traikov, Georgi, Teichert, Yosef, Wallenborn, Karl, Schütze, Wagner, El-Armouche, Tonn: "SARS-CoV-2 Infects Red Blood Cell Progenitors and Dysregulates Hemoglobin and Iron Metabolism." in: Stem cell reviews and reports, (2022) (PubMed).

  • 抗原

    SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

    别名

    Superoxide Dismutase 1

    途径

    Sensory Perception of Sound, Transition Metal Ion Homeostasis
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