抗体在线 Logo
电话:
+86 (0512) 65829739
传真:
+86 (010) 6788 5057
电子邮件:
orders@antibodies-online.cn

SOD1 抗体 (AA 2-154)

Cited in 1 publication. This 兔 多克隆 anti-SOD1 antibody specifically detects SOD1 in WB, IHC, IP 和 IF/ICC. The antibody is reactive with 人 和 马 samples. Independently validated for use in Western Blotting, Immunocytochemistry.
Pubmed (1 reference) Independent Validation (2 validations)
产品编号 ABIN2913314
发货至: 中国
Contact our Customer Service for availability and price in your country. Contact Info

Our Local Distributor

中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for SOD1 抗体 (AA 2-154) (ABIN2913314)

抗原

See all SOD1 抗体
SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

适用

  • 213
  • 132
  • 104
  • 39
  • 26
  • 16
  • 14
  • 14
  • 14
  • 12
  • 10
  • 9
  • 9
  • 9
  • 9
  • 9
  • 4
  • 4
  • 4
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
人, 马

宿主

  • 227
  • 41
  • 7
  • 4
  • 2
  • 2
  • 1
  • 1
兔

克隆类型

  • 203
  • 81
多克隆

标记

  • 113
  • 34
  • 23
  • 13
  • 9
  • 9
  • 7
  • 7
  • 7
  • 7
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 3
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This SOD1 antibody is un-conjugated

应用范围

  • 245
  • 146
  • 129
  • 76
  • 70
  • 59
  • 51
  • 17
  • 15
  • 14
  • 13
  • 7
  • 6
  • 4
  • 4
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (fixed cells) (IF/ICC)
Discover our top product SOD1 Primary Antibody

  • 抗原表位

    • 34
    • 22
    • 16
    • 16
    • 10
    • 8
    • 7
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 2-154

    纯化方法

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    免疫原

    SOD1 (Ala2-Pro154)

    亚型

    IgG
  • anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 1-154) antibody
    anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 1-154) antibody

    SOD1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

    anti-Superoxide Dismutase 1, Soluble (SOD1) (C-Term) antibody
    anti-Superoxide Dismutase 1, Soluble (SOD1) (C-Term) antibody

    KO Validated SOD1 适用: 人 WB, IHC (fro) 宿主: 兔 Polyclonal unconjugated

    anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 6-100) antibody
    anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 6-100) antibody

    SOD1 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, FACS, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated

    anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 2-154) antibody
    anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 2-154) antibody

    SOD1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

    anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 2-154) antibody
    anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 2-154) antibody

    SOD1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

    anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 2-153) antibody (APC)
    anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 2-153) antibody (APC)

    SOD1 适用: 兔 WB, IHC 宿主: 豚鼠 Polyclonal APC

    anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 2-154) antibody
    anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 2-154) antibody

    SOD1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

    anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 2-153) antibody
    anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 2-153) antibody

    SOD1 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

    anti-Superoxide Dismutase 1, Soluble (SOD1) antibody
    anti-Superoxide Dismutase 1, Soluble (SOD1) antibody

    SOD1 适用: 人, 小鼠 WB, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 6F5 unconjugated

    anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 55-84) antibody
    anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 55-84) antibody

    SOD1 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated

  • 应用备注

    Optimal working dilution should be determined by the investigator.

    限制

    仅限研究用
  • 生效 #100074 (Western Blotting)
    '独立验证'标志
    by
    Royal Veterinary College, London
    No.
    #100074
    日期
    2016.11.17
    抗原
    SOD-1
    Lot Number
    Method validated
    Western Blotting
    Positive Control
    Horse Fibroblasts, human SH-SY5Y
    Negative Control
    Notes
    ABIN3058918 successfully labels SOD-1 in lysates of equine fibroblast and human SH-SY5Y cells.
    '独立验证'标志
    Validation Images
    Total protein lysates from horse fibroblasts and human SH-SY5Y cells were separated were separated on a polyacrylamide gel under denaturing conditions. SOD-1 and TUBB protein bands were revealed as described in the protocol section. Total protein lysates from horse fibroblasts and human SH-SY5Y cells were separated were separated on a polyacrylamide gel under denaturing conditions. SOD-1 and TUBB protein bands were revealed as described in the protocol section.
    Full Methods
    Primary Antibody
    ABIN3058918
    Secondary Antibody
    Goat anti-rabbit-IgG HRP-linked whole antibody (Dako, D0487)
    Full Protocol
    • Seed 2x105 horse fibroblasts in a T25 flask and grow for 48h at 5% CO2 in supplemented with Dulbecco’s modified Eagle media (Sigma-Aldrich, D5546), with 10% heat-inactivated fetal calf serum (FCS) and 2mM (1%) L-glutamine and 1% penicillin (10IU/ml)/streptomycin (100µg/ml) to form a monolayer of 70-90%.
    • Human SH-SY5Y are cultured in identical conditions.
    • Wash cells once ice cold PBS.
    • Add 500µl chilled RIPA buffer (50mM Tris pH 7.5, 150mM NaCl, 1% NP40, 0.5% Na-deoxychlorate, 0.5% SDS, 1mM EDTA, 1mM EGTA, 1mM PMSF and 1x protease inhibitor) to each flask and incubate for 1min on ice.
    • Scrape each monolayer and transfer protein aggregates to a separate 1.5ml microcentrifuge tube.
    • Incubate for 20min on ice.
    • Centrifuge at 14000rpm for 20min at 4°C.
    • Remove the supernatant and determine the concentration of soluble proteins against a BSA protein standard using a DC protein assay (Biorad, 5000112).
    • Adjust protein samples to 0.3mg/ml total protein, using RIPA buffer and loading buffer.
    • After boiling for 5-10min, 1-10µg of each sample is separated on an Any kD Mini-PROTEAN TGX Stain-Free Precast Gel (Biorad, 4568123) at 100V for 90min.
    • Transfer proteins onto a PVDF blotting membrane (Hybond P, GE Healthcare Life Sciences, RPN303D) for 90min at 300mA.
    • Block the membrane with PBST containing 10% dry milk for 1h at RT.
    • Incubation with primary SOD-1 antibody (antibodies-online, ABIN3058918) diluted 1:1000 and a TUBB loading control antibody (abcam, ab6046) diluted 1:1000 in PBST containing 5% dry milk ON at 4°C.
    • Wash 5 times with PBST over 1 hour whilst shaking.
    • Incubation with secondary goat anti-rabbit-IgG horseradish peroxidase-linked whole antibody (Dako, D0487) diluted 1:5000 in PBST for 1h at RT.
    • Wash 5 times with PBST over 1 hour whilst shaking.
    • Blots were developed with Amersham ECL Prime Western Blotting Detection reagent (GE Healthcare Life Sciences, RPN2232) and visualized on a Kodak developer.
    Experimental Notes
    The same human cell lysates were also used for a different blot with an anti-human SOD-1 antibody (lab stock). A band of the same molecular weight as in the horse fibroblast lysates was revealed.
  • 生效 #100075 (Immunocytochemistry)
    '独立验证'标志
    by
    Royal Veterinary College, London
    No.
    #100075
    日期
    2016.11.17
    抗原
    SOD-1
    Lot Number
    Method validated
    Immunocytochemistry
    Positive Control
    Horse Fibroblasts
    Negative Control
    Notes
    ABIN3058918 achieves SOD-1 staining in horse fibroblasts when grown in a monolayer.
    '独立验证'标志
    Validation Images
    Horse fibroblasts were grown in a monolayer and stained with ABIN3058918 (red) as desecribed in the protocol. DAPI (blue) was used to visualize nuclei. Horse fibroblasts were grown in a monolayer and stained with ABIN3058918 (red) as desecribed in the protocol. DAPI (blue) was used to visualize nuclei.
    Full Methods
    Primary Antibody
    ABIN3058918
    Secondary Antibody
    anti-rabbit labeled with Alexa Fluor 594
    Full Protocol
    • Horse fibroblasts are seeded onto glass coverslips pre-treated with 1:10 diluted Matrigel (BD Biosciences, 354234) at a density of 1x105 cells per coverslip in a 24 well plate.
    • Incubate for 48h at 37°C in 5% CO2.
    • Wash coverslips with PBS.
    • Fix coverslips with 4% paraformaldehyde (PFA) and 0.1% triton for 20min at 4°C, followed by 8% PFA for 30min at RT.
    • Permeabilize cells with 0.5% triton for 30min at RT.
    • Wash coverslips 3 times with PBS.
    • Incubate coverslips with primary rabbit anti SOD-1 antibody (antibodies-online, ABIN3058918) diluted 1:40 in a dark, humidified chamber for 1h at RT. A negative control was incubated without primary antibody.
    • Wash coverslips 3 times with PBS.
    • Incubate with secondary goat anti rabbit labeled with Alexa Fluor 594 (ThermoFisher Scientific, A10239) diluted 1:500 for 1h at RT.
    • Wash coverslips 3 times with PBS.
    • Mount coverslips in a hard setting mount with VECTASHIELD HardSet Antifade Mounting Medium with DAPI (Vector Laboratories, H-1500).
    • Fluorescence microscopy is then used to image each coverslip.
    Experimental Notes

  • 状态

    Liquid

    浓度

    500 μg/mL

    缓冲液

    0.01M PBS,  pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

    储存液

    ProClin

    注意事项

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at 4 °C for frequent use. Aliquot and store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
  • Kronstein-Wiedemann, Stadtmüller, Traikov, Georgi, Teichert, Yosef, Wallenborn, Karl, Schütze, Wagner, El-Armouche, Tonn: "SARS-CoV-2 Infects Red Blood Cell Progenitors and Dysregulates Hemoglobin and Iron Metabolism." in: Stem cell reviews and reports, (2022) (PubMed).

  • 抗原

    SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

    别名

    Superoxide Dismutase 1

    途径

    Sensory Perception of Sound, Transition Metal Ion Homeostasis
You are here:
antibodies-online
德语

german (deutsch) antikoerper-online.de

英语

english (english) antibodies-online.com

法语

french (français) anticorps-enligne.fr

中文

chinese (中文) antibodies-online.cn

genomics-online
英语

english (english) genomics-online.com