Latexin 抗体 (AA 2-222)
Quick Overview for Latexin 抗体 (AA 2-222) (ABIN291079)
抗原
See all Latexin (LXN) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 2-222
-
特异性
- Selected for its ability to recognize mouse Latexin in direct ELISAs and Western Blots. In these formats, this antibody shows ~40 % cross-reactivity with rhLatexin.
-
纯化方法
- Affinity purified
-
免疫原
- E. coli-derived rmLatexin (aa2-222).
-
亚型
- IgG
-
-
anti-Latexin (LXN) (AA 1-223) antibody
LXN 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Latexin (LXN) antibodyLXN 适用: 人, 小鼠 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 2G4 unconjugated
anti-Latexin (LXN) (AA 151-222) antibodyLXN 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 8H5 unconjugated
anti-Latexin (LXN) antibodyKO Validated LXN 适用: 人 WB, ICC, IHC (p), IF 宿主: 兔 Polyclonal unconjugated
anti-Latexin (LXN) antibodyLXN 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB22193 unconjugated
anti-Latexin (LXN) (C-Term) antibodyVerified LXN 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 山羊 Polyclonal unconjugated
anti-Latexin (LXN) (Internal Region) antibodyLXN 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Latexin (LXN) (AA 1-216) antibodyLXN 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Latexin (LXN) (AA 1-222) antibodyLXN 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Latexin (LXN) (AA 1-222) antibodyLXN 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Approved: ELISA, IP, Neut, WB
Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse Latexin. The detection limit for rmLatexin is approximately 5 ng/lane and 0.5 ng/lane under non-reducing and reducing conditions, respectively. Immunoprecipitation: This antibody has been used to immunoprecipitate rmLatexin from conditioned media of transfected NS0 cells. Neutralization: This antibody has been used to neutralize the inhibitory activity of rmLatexin against rmCPA. The antibody was preincubated with the inhibitor at different molar ratios at room temperature for 30 minutes. The resulting mixtures were then incubated with the enzyme at room temperature for 60 minutes. The enzyme was assayed with a peptide substrate. 50 % of the proteolytic activity was restored by the antibody at 4.4 μg/mL (IC50) under conditions in which 0.1 μg/mL of the inhibitor, 0.1 μg/mL of the enzyme, and 100 M of the substrate were present. Considering the molecular masses of the inhibitor (26kD) and the antibody (150kD), IC50 was achieved at approximately 8:1 Molar ratio of the antibody to the inhibitor. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse Latexin. The detection limit for rmLatexin is approximately 0.3 ng/well. -
说明
-
Target Species of Antibody: Mouse
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- deionized water
-
浓度
- Lot specific
-
缓冲液
- Lyophilized.
-
-
- Latexin (LXN)
-
别名
- Latexin / MUM
-
背景
-
Name/Gene ID: LXN
Synonyms: LXN, ECI, MUM, Protein MUM, TCI, Latexin -
基因ID
- 56925
-
UniProt
- Q9BS40
抗原
-
