UCP1 抗体 (AA 179-296)
(1 validation)Quick Overview for UCP1 抗体 (AA 179-296) (ABIN2905048)
抗原
See all UCP1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 179-296
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纯化方法
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
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免疫原
- UCP1 (Pro179-Leu296)
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anti-Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1) (AA 1-307) antibody
UCP1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1) (AA 101-200) antibodyUCP1 适用: 人, 小鼠, 大鼠, 山羊 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1) (AA 179-296) antibodyUCP1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1) (AA 2-307) antibodyUCP1 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1) (AA 1-307) antibodyUCP1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1) (AA 1-307) antibodyUCP1 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1) (C-Term) antibodyVerified UCP1 适用: 大鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1) (AA 232-267) antibodyUCP1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4B7 unconjugated
anti-Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1) (AA 179-296) antibodyUCP1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1) (AA 232-267) antibodyUCP1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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- by
- Mivechi lab, Georgia Cancer Center, Augusta University
- No.
- #103357
- 日期
- 2018.10.03
- 抗原
- UCP1
- Lot Number
- AB1808U769
- Method validated
- Western Blotting
- Positive Control
Cold exposed mouse brown adipose tissue (BAT)
- Negative Control
Mouse liver tissue
- Notes
Passed. ABIN3056733 can detect mouse UCP1 protein in immunoblotting analysis.
- Primary Antibody
- ABIN3056733
- Secondary Antibody
- donkey anti-rabbit HRP-conjugated antibody (Jackson Immuno Research, 711-035-152)
- Full Protocol
- Homogenize mouse brown fat tissue in 2ml mitochondria isolation buffer (70mM sucrose, 210mM mannitol, 5mM HEPES, 1mM EGTA, pH7.2, and 0.5% BSA).
- Centrifuge the homogenates for 10min at 800g at 4°C.
- Centrifuge the supernatant again at 8000g for 10min at 4°C.
- Lyse the resulting mitochondria fraction in RIPA buffer containing protease inhibitor cocktail (Pierce Protease Inhibitor, A32965, lot TA2492391).
- Determined protein concentration with Pierce BCA Protein Assay Kit (Pierce, 23225, lot OA180049).
- Separate 50µg of total protein per lane on a denaturing 12% SDS-PAGE gel (Bio-Rad, 1610156, lot 64144809) in a Mini Protean Tetra Cell for 2h at 80V.
- Transfer proteins onto Immobilon-P PVDF Membrane (Millipore, IPVH00010) using Mini Trans Blot Cell system (Bio-Rad, 1703930).
- Block membrane with 5% milk in PBS-T for 1h at RT.
- Incubate membrane with primary rabbit anti-UCP1 antibody (antibodies-online, ABIN3056733, lot AB1808U769) diluted to 1µg/ml ON at 4°C.
- Wash membrane 3x for 10min with PBS-T.
- Incubate membrane with secondary donkey anti-rabbit HRP-conjugated antibody (Jackson Immuno Research, 711-035-152) for 1h at RT.
- Wash membrane 3x for 10min with PBS-T.
- Incubate membrane Pierce ECL Western Blotting Substrate working solution (Thermo Scientific, 1859701, lot TB263310) for 1min at RT. Expose membrane to x-ray film for different amount of time.
- Experimental Notes
The UCP1 antibody ABIN3056733 reveals a specific protein band in positive control samples (cold exposed mouse brown fat tissue), but not in negative control samples (liver tissue).
生效 #103357 (Western Blotting)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Lysates from mitochondrial fraction isolated from mouse liver tissue (negative control) or brown adipose tissue (BAT) without (22°C) or with (4°C) cold exposure for 6h were resolved by SDS-PAGE gel electrophoresis followed by Western blotting analysis using UCP1 primary antibody ABIN3056733.]()
Lysates from mitochondrial fraction isolated from mouse liver tissue (negative control) or brown adipose tissue (BAT) without (22°C) or with (4°C) cold exposure for 6h were resolved by SDS-PAGE gel electrophoresis followed by Western blotting analysis using UCP1 primary antibody ABIN3056733.
Full Methods -
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状态
- Liquid
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浓度
- 500 μg/mL
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缓冲液
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4 °C for frequent use. Aliquot and store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
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- UCP1 (Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1))
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别名
- Uncoupling Protein 1, Mitochondrial
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UniProt
- P12242
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途径
- Proton Transport, Brown Fat Cell Differentiation
抗原
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