ThIS Rat UCP4 peptide sequence is 100 % conserved in alternatively spliced isoforms a-c of rat, 85 % in human, and 71 % in mouse UCP4. UCP42-P has no significant homology with UCP1-3 or UCP-5.
纯化方法
Affinity chromatography purified
免疫原
UCP4 antibody was raised in rabbit using a 14 amino acid peptide from rat UCP4 as the immunogen.
ELISA: 1:10,000-1:100,000, HC: 2-20 µg/mL, IF: 2-20 µg/mL, WB: 1-10 µg/mL Optimal conditions should be determined by the investigator.
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstitute in water to a concentration of 1 mg/mL. Do not dilute further until ready to use.
浓度
Lot specific
缓冲液
Affinity purified IgG lyophilized from 100 mM Tris, pH 7.5, with 0.02 % BSA, and 0.05 % NaN3.
储存液
Sodium azide
注意事项
This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
注意事项
Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
储存条件
4 °C/-20 °C
储存方法
Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.
抗原
SLC25A27
(Solute Carrier Family 25, Member 27 (SLC25A27))
别名
UCP4
背景
Uncoupling proteins (UCP1-5) are a family of mitochondria transport proteins that play a critical role in thermoregulatory heat production and maintenance of basal metabolic rate. UCP3 is predicted to contain 6 transmembrane domains. UCP4 (mouse/rat 322 aa, human 323 aa, chromosome 6p11.2-q12) is expressed in both fetal and adult brain tissues.