This rat UCP3 peptide sequence is 100 % conserved in mouse, 94 % in hamster, 66 % in canine, 61 % in bovine, 63 % in monkey, and 38 % in human UCP3. It has no significant homology with UCP1-2 or UCP4-5.
纯化方法
Affinity chromatography purified
免疫原
UCP3 antibody was raised in rabbit using an 18 amino acid peptide from rat UCP3 as the immunogen.
ELISA: 1:10,000-1:100,000, HC: 2-20 µg/mL, IF: 2-20 µg/mL, WB: 1-10 µg/mL Optimal conditions should be determined by the investigator.
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstitute in PBS to 1 mg/mL.
浓度
Lot specific
缓冲液
Lyophilized from PBS with 0.1 % BSA, and 0.05 % NaN3.
储存液
Sodium azide
注意事项
This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
注意事项
Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
储存条件
4 °C/-20 °C
储存方法
Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.
抗原
UCP3
(Uncoupling Protein 3 (Mitochondrial, Proton Carrier) (UCP3))
别名
UCP3
背景
Uncoupling proteins (UCP1-5) are a family of mitochondria transport proteins that play a critical role in thermoregulatory heat production and maintenance of basal metabolic rate. UCP3 is predicted to contain 6 transmembrane domains. The UCP3S lacks the 6th transmembrane domain and it is only 275 aa long. UCP3 has preferential expression in muscle and it is unaffected by cold acclimation.