Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Western Blotting (WB)
交叉反应 (详细)
Peptide sequence is <50% identical to other chemokine receptors in this regoin and cross-reactivity to mouse CCR D6 was .
纯化方法
Purified
免疫原
CCRD6 antibody was raised in goat using a synthetic peptide C-L10 ATEDADSENSSFYYYDYLDEVAFM30 corresponding to the N-terminal extracellular domain of human D6 as the immunogen.
ELISA: 1:50,000, FC: 2-4 µL/10^6 cells/50-104 µL, IHC: 1:125, WB: 1:500 Optimal conditions should be determined by the investigator.
限制
仅限研究用
浓度
Lot specific
缓冲液
Affinity urified and supplied in 10 mM KHPO4, pH 7.2, with 140 M NaCl, 1 mg/mL BSA and 0.1 % NaN3.
储存液
Sodium azide
注意事项
This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
注意事项
Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
储存条件
-20 °C/-80 °C
储存方法
Aliquot and store at -20 °C for short term storage, -70 °C for long-term storage.
抗原
CCRD6
背景
CCRD6 is a beta chemokine receptor similar to G protein-coupled receptors. Chemokines and the receptor-mediated signal transduction are critical for the recruitment of effector immune cells to the inflammation site. This receptor appears to bind the majority of beta-chemokine family members, however, its specific function remains unkown.