Ig 抗体 (Light Chain) (HRP)
This anti-Ig antibody is a 山羊 多克隆 antibody detecting Ig in ELISA, WB, IHC 和 ICC. Suitable for 小鼠.
Quick Overview for Ig 抗体 (Light Chain) (HRP) (ABIN288438)
抗原
See all Ig 抗体
Ig
适用
小鼠
宿主
山羊
克隆类型
多克隆
标记
This Ig antibody is conjugated to HRP
应用范围
ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
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抗原表位
- Light Chain
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特异性
- This antibody reacts with Human, Mouse, and Rat. This antibody does not cross-react with GLUT-4.
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交叉反应 （详细）
- This Antibody may cross react with lambda from other species1 ml@ 1 mg/ml
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纯化方法
- Affinity chromatography purified
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亚型
- IgG
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anti-Ig antibody (PE)
适用: 兔 ELISA, FACS, FLISA 宿主: 大鼠 Monoclonal SB87a PE
anti-Ig antibody适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1:10,000-1:100,000, ICC: 1:200-1:500, IHC: 1:200-1:500, WB: colorimetric: 1:1,000-1:10,000, chemiluminescent: 1:1,000-1:30,000
Optimal conditions should be determined by the investigator. -
限制
- 仅限研究用
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浓度
- Lot specific
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缓冲液
- 50 mM Hepes, pH 7.1, with 0.0 NaCl, 0.2 % BSA, and 0.01 % Thimerosal.
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储存液
- Thimerosal (Merthiolate)
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注意事项
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
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Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Dilute only prior to immediate use. -
储存条件
- 4 °C
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- Ig
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背景
- There are two types of light chain in mammals, the kappa chain, encoded by the immunoglobulin kappa locus on chromosome 2, and the lambda chain, encoded by the immunoglobulin lambda locus on chromosome 22. Antibodies are produced by B lymphocytes, each expressing only one class of light chain. Once set, light chain class remains fixed for the life of the B lymphocyte.
抗原
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