Alkaline Phosphatase 抗体 (HRP)
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Quick Overview for Alkaline Phosphatase 抗体 (HRP) (ABIN288216)
抗原
See all Alkaline Phosphatase (ALP) 抗体适用
宿主
克隆类型
标记
应用范围
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交叉反应 (详细)
- No cross reactivity against mammalian forms of alkaline Phosphatase.
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纯化方法
- Affinity chromatography purified
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免疫原
- Alkaline phosphatase antibody (HRP) was raised in rabbit using alkaline phosphatase isolated from E.coli as the immunogen.
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应用备注
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ELISA: 1:4,000-1:20,000, WB: 1:500-1:2,000
Optimal conditions should be determined by the investigator. -
限制
- 仅限研究用
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状态
- Lyophilized
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浓度
- Lot specific
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缓冲液
- Lyophilized from 0.02 M K2 O4, pH 7.2, with 0.12 NaCl, 10 mg/mL BSA, and 0.01 % gentamycin sulfate. DO NOT add Sodium azide. Immunoglobulin and protease free.
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注意事项
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Avoid repeated freeze/thaw cycles.
Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Dilute only prior to immediate use. -
储存条件
- 4 °C/-20 °C
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储存方法
- Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.
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- Alkaline Phosphatase (ALP)
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别名
- Alkaline Phosphatase
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背景
- Alkaline phosphatase is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. As the name suggests, alkaline phosphatases are most effective in an alkaline environment. It is sometimes used synonymously as basic phosphatase.
抗原
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