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Alkaline Phosphatase 抗体 (HRP)

The HRP-conjugated 兔 多克隆 anti-Alkaline Phosphatase antibody (ABIN288216) specifically detects Alkaline Phosphatase in WB 和 ELISA. The antibody is reactive with E. coli samples.
产品编号 ABIN288216
发货至: 中国
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Quick Overview for Alkaline Phosphatase 抗体 (HRP) (ABIN288216)

抗原

See all Alkaline Phosphatase (ALP) 抗体
Alkaline Phosphatase (ALP)

适用

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E. coli

宿主

  • 93
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克隆类型

  • 81
  • 45
多克隆

标记

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This Alkaline Phosphatase antibody is conjugated to HRP

应用范围

  • 85
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Western Blotting (WB), ELISA
  • 交叉反应 (详细)

    No cross reactivity against mammalian forms of alkaline Phosphatase.

    纯化方法

    Affinity chromatography purified

    免疫原

    Alkaline phosphatase antibody (HRP) was raised in rabbit using alkaline phosphatase isolated from E.coli as the immunogen.
  • 应用备注

    ELISA: 1:4,000-1:20,000, WB: 1:500-1:2,000
    Optimal conditions should be determined by the investigator.

    限制

    仅限研究用
  • 状态

    Lyophilized

    浓度

    Lot specific

    缓冲液

    Lyophilized from 0.02 M K2 O4, pH 7.2, with 0.12 NaCl, 10 mg/mL BSA, and 0.01 % gentamycin sulfate. DO NOT add Sodium azide. Immunoglobulin and protease free.

    注意事项

    Avoid repeated freeze/thaw cycles.
    Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
    Dilute only prior to immediate use.

    储存条件

    4 °C/-20 °C

    储存方法

    Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.
  • 抗原

    Alkaline Phosphatase (ALP)

    别名

    Alkaline Phosphatase

    背景

    Alkaline phosphatase is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. As the name suggests, alkaline phosphatases are most effective in an alkaline environment. It is sometimes used synonymously as basic phosphatase.
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