Streptavidin 抗体 (FITC)
Quick Overview for Streptavidin 抗体 (FITC) (ABIN7121097)
抗原
See all Streptavidin 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Ion exchange chromatography
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免疫原
- Streptavidin antibody (FITC) was raised in rabbit using streptavidin as the immunogen.
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anti-Streptavidin (AA 25-183) antibody
适用: Streptomyces avidinii ELISA, WB 宿主: 小鼠 Monoclonal 1C2 unconjugated
anti-Streptavidin (C-Term) antibody适用: Streptomyces avidinii WB 宿主: 山羊 Polyclonal unconjugated
anti-Streptavidin (AA 25-183) antibody适用: Streptomyces avidinii ELISA, WB 宿主: 小鼠 Monoclonal 1C2 unconjugated
anti-Streptavidin antibody适用: Streptomyces avidinii ELISA, WB, IHC, DB 宿主: 兔 Polyclonal unconjugated
anti-Streptavidin antibody (Texas Red (TR))适用: Streptomyces avidinii FACS, FM, FLISA 宿主: 兔 Polyclonal Texas Red (TR)
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应用备注
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FC: 1:2,000-1:10,000, IF: 1:500-1:2,500, OTHER: FC: 1:2,000-1:10,000
Optimal conditions should be determined by the investigator. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- IgG fraction supplied as a liquid in 20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.2, with 10 mg/mL BSA (IgG and Protease free) as a stabilizer, and 0.01 % NaN3 as a preservative.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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注意事项
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Avoid repeated freeze/thaw cycles.
Product is photosensitive and should be protected from light.
Dilute only prior to immediate use. -
储存条件
- 4 °C/-20 °C
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储存方法
- Store at 4 °C or aliquot and freeze at -20 °C for long term storage.
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- Streptavidin
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背景
- Streptavidin is a 52,800 dalton tetrameric protein purified from the bacterium Streptomyces avidinii. It has an extraordinarily high affinity for biotin (also known as vitamin B7), the dissociation constant (kDa) of the biotin-streptavidin complex is on the order of 10-14 mol/L, making it one of the strongest non-covalent interactions known in nature. It is used extensively in molecular biology and bionanotechnology as, in addition to the high affinity, biotin-binding is resistant to extremes of pH, temperature, organic solvents, denaturants, detergents and proteolytic enzymes.
抗原
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