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Choline Acetyltransferase 抗体 (N-Term) (FITC)

This anti-Choline Acetyltransferase antibody is a 兔 多克隆 antibody detecting Choline Acetyltransferase in WB, IF 和 ICC. Suitable for 人.
产品编号 ABIN2869106
发货至: 中国

Quick Overview for Choline Acetyltransferase 抗体 (N-Term) (FITC) (ABIN2869106)

抗原

See all Choline Acetyltransferase (CHAT) 抗体
Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

适用

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宿主

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克隆类型

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多克隆

标记

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This Choline Acetyltransferase antibody is conjugated to FITC

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
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    N-Term

    特异性

    Predicted molecular weight at ~82.5 kDa. Observed molecular weights between 68-70 kDa.

    交叉反应

    人, 小鼠

    纯化方法

    Peptide Affinity Purified

    免疫原

    Synthetic peptide from the N-terminal to the mid-protein of human Choline O-Acetyltrasferase
  • 应用备注

    • WB (1:1000)
    • ICC/IF (1:100)
    • optimal dilutions for assays should be determined by the user.

    说明

    A 1:1000 dilution of ABIN2869106 was sufficient for detection of Choline Acetyltransferase on mouse brain lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Conjugated antibodies should be stored at 4°C
  • 抗原

    Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

    别名

    Choline Acetyltransferase

    背景

    Acetylcholine (ACh) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransferase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-choline acetyltransferase a useful specific marker. ChAT is a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer disease and Down syndrome (1-3).

    基因ID

    1103

    NCBI登录号

    NP_001136401

    UniProt

    P28329

    途径

    Skeletal Muscle Fiber Development
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