Shiga-like Toxin (Stx) (Subunit A) 抗体
This anti- antibody is a 小鼠 单克隆 antibody detecting in EIA 和 Neut. Suitable for E. coli.
Quick Overview for Shiga-like Toxin (Stx) (Subunit A) 抗体 (ABIN2859493)
抗原
Shiga-like Toxin (Stx)
适用
E. coli
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Enzyme Immunoassay (EIA), Neutralization (Neut)
克隆位点
557
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抗原表位
- Subunit A
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纯化方法
- Protein A affinity chromatography
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纯度
- > 90 % pure
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亚型
- IgG2
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应用备注
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ELISA. Neutralization.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.1 mg/mL ( OD280nm, E0.1% = 1.4)
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缓冲液
- 0.01 M PBS, pH 7.2, containing 0.09 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Upon receipt, aliquot and store at -20 °C.
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- Shiga-like Toxin (Stx)
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背景
- Verotoxins are produced by certain enterohaemorrhagic strains of Escherichia coli. Serotype O157:H7 has been the strain most commonly associated with large food bourne outbreaks and has been implicated in a wide spectrum of diseases including blood diarrhoea, haemorrhagic colitis and haemolytic uraemic syndrome. The main sources of infection are contaminated foods of animal origin such as meat and dairy products. Verotoxins, also called Shiga like toxins( SLT) because of their similarity to toxins produced by Shigella dysenteriae, are classified into two main categories: verotoxin 1 (VT1, SLT1, Stx1) and 2 (VT2, SLT2, Stx2). Verotoxin production is the most common criteria for the detection of this group of bacteria rather than tests for the cytopathic effect on Vero cells that gave the toxins their name. Verotoxins contain a single A subunit and multiple copies of a B subunit. The A subunit is responsible for inhibiting protein synthesis through the catalytic inactivation of 60S ribosomal subunits.Synonyms: SLT-2 A subunit, SLT-2a, SLT-IIa, Verocytotoxin 2 subunit A, Verotoxin 2 subunit A, rRNA N-glycosidase 2, stx2A, stxA2
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基因ID
- 10730
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UniProt
- P09385
抗原
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