VRN2 抗体
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北京 101111
Quick Overview for VRN2 抗体 (ABIN7477853)
抗原
适用
宿主
克隆类型
应用范围
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原理
- Anti-VRN2 | Vernalization protein 2
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交叉反应 （详细）
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Triticum aestivum
Not reactive in: No confirmed exceptions from predicted reactivity are currently known -
预测反应
- Triticum monococcum
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纯化方法
- Protein G purified
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免疫原
- KLH-conjugated synthetic peptide chosen from Triticum aestivum VRN2 sequence
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应用备注
- 1 : 10 000 (ELISA), 1 : 1000 (WB)
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说明
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there is some non-specific binding in a western blot when using anti-TaVRN2 antibody on nuclear extracts of T. monococcum
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 50 μL of sterile water
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缓冲液
- Total IgG. Protein G purified purified from Cell culture supernatant.
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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- VRN2 (Polycomb Group Protein VERNALIZATION 2 (VRN2))
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别名
- VRN2
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背景
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Background: Plants with a winter growth habit flower earlier when exposed for several weeks to cold temperatures, a process called vernalization. The wheat vernalization gene VRN-2 is a dominant repressor of flowering that is down-regulated by vernalization. Loss of function of VRN-2, whether by natural mutations or deletions, results in spring lines, which do not require vernalization to flower.
Additional information: VRN-2 and ZCCT2 full-length proteins were expressed as GST fusion proteins in E. coli and purified through GST sepharose columns. The purified VRN-2 and ZCCT2 proteins were used to test the specificity of the VRN-2 antibody by Western blot analysis. A Western blot experiment showed that the VRN-2 antibody was able to differentiate VRN-2 from the ZCCT2 protein.
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分子量
- 23.7 kDa
抗原
-
