Pyruvate Kinase 抗体
Quick Overview for Pyruvate Kinase 抗体 (ABIN285893)
抗原
See all Pyruvate Kinase (PK) 抗体适用
宿主
克隆类型
标记
应用范围
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交叉反应 (详细)
- Reacts with all forms of pyruvate kinase (pan M-PK). Cross reactivity agains Pyruvate Kinase from other mammalian tissues is expected but has not been specifically determined.
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纯化方法
- Ion exchange chromatography
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免疫原
- Pyruvate kinase antibody (IgG fraction) was raised in goat using pyruvate kinase isolated from rabbit Muscle as the immunogen.
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亚型
- IgG
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应用备注
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ELISA: 1:4,000-1:20,000, IHC: 1:500-1:2,000, WB: 1:500-1:2,000
Optimal conditions should be determined by the investigator. -
限制
- 仅限研究用
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状态
- Lyophilized
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浓度
- Lot specific
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缓冲液
- Lyophilized from 0.02 M K2 O4, pH 7.2, with 0.12 NaCl, and 0.01 % NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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注意事项
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Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use. -
储存条件
- 4 °C/-20 °C
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储存方法
- Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.
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- Pyruvate Kinase (PK)
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别名
- Pyruvate Kinase
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背景
- Pyruvate kinase is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP.
抗原
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