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VCP 抗体 (N-Term)

Name: VCP 抗体 (N-Term)
Brand: antibodies-online
SKU: ABIN2856882
Availability: OutOfStock

This anti-VCP antibody is a 兔多克隆 antibody detecting VCP in WB, IF, IP, IHC (p), ICC 和 IHC (wm). Suitable for 人. This Primary Antibody has been cited in 3+ publications.

WB Image VCP antibody detects VCP protein by Western blot analysis. (VCP 抗体 (N-Term))

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using VCP(p97), antibody at 1:200 dilution. (VCP 抗体 (N-Term))

WB Image Sample (50 ug of whole cell lysate) A: mouse brain 7. (VCP 抗体 (N-Term))

IHC-P Image VCP antibody [N1N2], N-term detects VCP protein at nucleus and cytosol on rat brain stem by immunohistochemical analysis. (VCP 抗体 (N-Term))

IHC

IHC-P Image Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using VCP(p97), antibody at 1:500 dilution. (VCP 抗体 (N-Term))

IHC

WB Image Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 , C: Molt-4 , D: Raji 7. (VCP 抗体 (N-Term))

PME-1 knockout reduces whole cell PP2A activity. (VCP 抗体 (N-Term))

PME-1 knockout reduces PP2A protein levels. (VCP 抗体 (N-Term))

8 images

(3 references)

产品编号 ABIN2856882

发货至: 中国

Quick Overview for VCP 抗体 (N-Term) (ABIN2856882)

抗原

See all VCP 抗体

VCP (Valosin Containing Protein (VCP))

适用

人

宿主

兔

克隆类型

多克隆

标记

This VCP antibody is un-conjugated

应用范围

Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Whole Mount) (IHC (wm))

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N-Term

交叉反应

犬, 人, 小鼠, 大鼠, 斑马鱼

产品特性

Rabbit Polyclonal antibody to VCP (valosin-containing protein)
VCP antibody [N1N2], N-term

纯化方法

Purified by antigen-affinity chromatography.

免疫原

Recombinant protein encompassing a sequence within the N-terminus region of human VCP. The exact sequence is proprietary.

亚型

IgG
anti-Valosin Containing Protein (VCP) (C-Term) antibody
KO Validated VCP 适用: 人 WB, IF, IP, IHC (p), ICC, IHC (fro), IHC (wm) 宿主: 兔 Polyclonal unconjugated

anti-Valosin Containing Protein (VCP) (AA 125-371) antibody
VCP 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Valosin Containing Protein (VCP) (AA 1-588) antibody
VCP 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 4A8 unconjugated

anti-Valosin Containing Protein (VCP) (AA 647-806) antibody
VCP 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Valosin Containing Protein (VCP) antibody
VCP 适用: 人 WB, IHC, ELISA, IF, IP 宿主: 兔 Monoclonal 5H12 unconjugated Recombinant Antibody

anti-Valosin Containing Protein (VCP) antibody
VCP 适用: 人, 小鼠 WB, FACS, ICC 宿主: 小鼠 Monoclonal Hs-14 unconjugated

anti-Valosin Containing Protein (VCP) (AA 749-766), (C-Term) antibody
VCP 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, IF, FACS, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Valosin Containing Protein (VCP) (AA 732-760), (C-Term) antibody
VCP 适用: 人, 小鼠, 大鼠 WB, IHC, IF, FACS, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Valosin Containing Protein (VCP) (AA 732-760) antibody
VCP 适用: 人, 小鼠, 大鼠 WB, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Valosin Containing Protein (VCP) (C-Term) antibody
VCP 适用: 人, 小鼠, 大鼠 WB, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

应用备注

WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

说明

Positive Control: Mouse brain , rat brain , A549 , H1299 , HCT116

限制

仅限研究用
状态

Liquid

浓度

0.3 mg/mL

缓冲液

1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Tsuji, Ohama, Nakagawa, Sato: "Efficacy of an anti-cancer strategy targeting SET in canine osteosarcoma." in: The Journal of veterinary medical science, Vol. 81, Issue 10, pp. 1424-1430, (2020) (PubMed).

Kake, Tsuji, Enjoji, Hanasaki, Hayase, Yabe, Tanaka, Nakagawa, Liu, Chang, Usui, Ohama, Sato: "The role of SET/I2PP2A in canine mammary tumors." in: Scientific reports, Vol. 7, Issue 1, pp. 4279, (2019) (PubMed).

Yabe, Tsuji, Mochida, Ikehara, Usui, Ohama, Sato: "A stable association with PME-1 may be dispensable for PP2A demethylation - implications for the detection of PP2A methylation and immunoprecipitation." in: FEBS open bio, Vol. 8, Issue 9, pp. 1486-1496, (2018) (PubMed).
抗原

VCP (Valosin Containing Protein (VCP))

别名

valosin containing protein

分子量

89 kDa

基因ID

7415

UniProt

P55072

途径

ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Ubiquitin Proteasome Pathway