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Fatty Acid Synthase 抗体 (N-Term)

Name: Fatty Acid Synthase 抗体 (N-Term)
Brand: antibodies-online
SKU: ABIN2856754
Availability: OutOfStock

KO Validated FASN 适用: 人 WB, IP, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated

WB Image Sample (50 ug of whole cell lysate) A: mouse liver 5% SDS PAGE antibody diluted at 1:1000 (Fatty Acid Synthase 抗体 (N-Term))

IP Image Immunoprecipitation of Fatty Acid Synthase protein from HeLa whole cell extracts using 5 μg of Fatty Acid Synthase antibody [N1N2], N-term, Western blot analysis was performed using Fatty Acid Synthase antibody [N1N2], N-term, EasyBlot anti-Rabbit IgG was used as a secondary reagent. (Fatty Acid Synthase 抗体 (N-Term))

IHC-P Image Immunohistochemical analysis of paraffin-embedded OECM1 xenograft, using Fatty Acid Synthase, antibody at 1:500 dilution. (Fatty Acid Synthase 抗体 (N-Term))

IHC

WB Image Sample (30 ug of whole cell lysate) A: Molt-4 , 5% SDS PAGE antibody diluted at 1:1000 (Fatty Acid Synthase 抗体 (N-Term))

ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Fatty Acid Synthase, antibody (Green) at 1:500 dilution. (Fatty Acid Synthase 抗体 (N-Term))

ICC/IF Image Fatty Acid Synthase antibody [N1N2], N-term detects Fatty Acid Synthase protein at cytoplasm by immunofluorescent analysis. (Fatty Acid Synthase 抗体 (N-Term))

WB Image Fatty Acid Synthase antibody detects Fatty Acid Synthase protein by western blot analysis. (Fatty Acid Synthase 抗体 (N-Term))

7 images

产品编号 ABIN2856754

发货至: 中国

Datasheet as PDF

抗原 See all Fatty Acid Synthase (FASN) 抗体

Fatty Acid Synthase (FASN)
抗原表位
15

6

5

5

5

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
N-Term
适用
73

40

34

16

4

3

2

1

1

1

1

1

1

1

1
人
宿主
58

19

2
兔
Compatible Secondaries
克隆类型
53

26
多克隆
标记
54

3

3

2

2

2

2

2

2

2

1

1

1

1

1
This Fatty Acid Synthase antibody is un-conjugated
应用范围
63

24

21

20

20

16

14

14

13

5

4

3

2

1
Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

交叉反应

人, 小鼠

产品特性

Rabbit Polyclonal antibody to Fatty Acid Synthase (fatty acid synthase)
Fatty Acid Synthase antibody [N1N2], N-term

纯化方法

Purified by antigen-affinity chromatography.

质量等级

KO Validated

免疫原

Recombinant protein encompassing a sequence within the N-terminus region of human Fatty Acid Synthase. The exact sequence is proprietary.

亚型

IgG

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anti-Fatty Acid Synthase (FASN) (AA 2212-2511) antibody
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anti-Fatty Acid Synthase (FASN) (N-Term) antibody
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anti-Fatty Acid Synthase (FASN) (AA 942-973) antibody
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anti-Fatty Acid Synthase (FASN) (AA 2243-2505) antibody
FASN 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Fatty Acid Synthase (FASN) (AA 1-226) antibody
FASN 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Fatty Acid Synthase (FASN) (AA 651-750) antibody
FASN 适用: 人, 小鼠, 大鼠, 山羊 WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Fatty Acid Synthase (FASN) (AA 2155-2495) antibody
FASN 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

应用备注

WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

说明

Positive Control: Molt-4 , 293T , A431 , HeLa , HepG2
Validation: KO/KD, Orthogonal

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

储存液

Thimerosal (Merthiolate)

注意事项

This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
抗原

Fatty Acid Synthase (FASN)

别名

fatty acid synthase (FASN 产品)

别名

FAS antibody, OA-519 antibody, SDR27X1 antibody, FASN antibody, fj34h12 antibody, wu:fj34h12 antibody, Cgl0836 antibody, A630082H08Rik antibody, BcDNA:GH07626 antibody, BcDNA:gh07626 antibody, DM_7295848 antibody, Dmel\\CG3523 antibody, Fatty acid synthase antibody, anon-EST:Posey235 antibody, anon-EST:Posey43 antibody, anon-WO0138581.1 antibody, dFAS antibody, fas antibody, fatty acid synthase antibody, fatty acid synthase Fas antibody, type I polyketide synthase antibody, Fatty acid synthase Fas antibody, Fatty Acid SyNthase antibody, fatty-acid synthase antibody, fatty-acid synthase II antibody, fatty-acid synthase fas antibody, Fatty acid synthase 1 antibody, FASN antibody, fas antibody, Fasn antibody, BAD_RS01360 antibody, fasn antibody, fasn-1 antibody, NCgl0802 antibody, fasB antibody, MT2600 antibody, TBMG_01448 antibody, FASN1 antibody

背景

The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.

Cellular Localization: Cytoplasm , Melanosome

分子量

273 kDa

基因ID

2194

UniProt

P49327

途径

AMPK Signaling