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TJP1 抗体 (N-Term)

Cited in 1 publication. This anti-TJP1 antibody is a 兔 多克隆 antibody detecting TJP1 in WB, IP, IF 和 ICC. Suitable for 人.
产品编号 ABIN2856624
发货至: 中国
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Quick Overview for TJP1 抗体 (N-Term) (ABIN2856624)

抗原

See all TJP1 抗体
TJP1 (Tight Junction Protein 1 (TJP1))

适用

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宿主

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克隆类型

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多克隆

标记

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This TJP1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

质量等级

KO Validated
  • 抗原表位

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    N-Term

    交叉反应

    人, 小鼠

    产品特性

    Rabbit Polyclonal antibody to ZO-1 (tight junction protein 1 (zona occludens 1))
    ZO-1 antibody [N1N2], N-term

    纯化方法

    Purified by antigen-affinity chromatography.

    免疫原

    Recombinant protein encompassing a sequence within the N-terminus region of human ZO-1. The exact sequence is proprietary.

    亚型

    IgG
  • 应用备注

    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

    说明

    Positive Control: HeLa

    Validation: KO/KD

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.22 mg/mL

    缓冲液

    1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

    储存液

    ProClin

    注意事项

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Zhang, Heruth, Wu, Zhang, Nsumu, Shortt, Li, Jiang, Wang, Friesen, Li, Ye: "Functional characterization of SLC26A3 c.392C>G (p.P131R) mutation in intestinal barrier function using CRISPR/CAS9-created cell models." in: Cell & bioscience, Vol. 9, pp. 40, (2019) (PubMed).

  • 抗原

    TJP1 (Tight Junction Protein 1 (TJP1))

    别名

    tight junction protein 1

    背景

    This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene.

    Cellular Localization: Cell membrane, Peripheral membrane protein, Cytoplasmic side , Cell junction , tight junction

    分子量

    195 kDa

    基因ID

    7082

    UniProt

    Q07157

    途径

    Carbohydrate Homeostasis, Cell-Cell Junction Organization
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