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VPS35 抗体 (C-Term)

Cited in 1 publication. This 兔 多克隆 anti-VPS35 antibody specifically detects VPS35 in WB, IF, ICC 和 IHC (p). The antibody is reactive with 人, 小鼠 和 大鼠 samples.
产品编号 ABIN2856562
发货至: 中国
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Quick Overview for VPS35 抗体 (C-Term) (ABIN2856562)

抗原

See all VPS35 抗体
VPS35 (Vacuolar Protein Sorting 35 (VPS35))

适用

  • 112
  • 41
  • 25
  • 1
  • 1
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  • 1
  • 1
  • 1
  • 1
人, 小鼠, 大鼠

宿主

  • 56
  • 55
  • 1

克隆类型

  • 84
  • 28
多克隆

标记

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  • 5
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This VPS35 antibody is un-conjugated

应用范围

  • 90
  • 56
  • 55
  • 52
  • 51
  • 14
  • 9
  • 2
Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 抗原表位

    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    C-Term

    交叉反应

    斑马鱼, 小鸡, 牛

    交叉反应 (详细)

    Zebrafish (86 %), Chicken (93 %), Bovine (100 %)

    产品特性

    Rabbit Polyclonal antibody to VPS35 (vacuolar protein sorting 35 homolog (S. cerevisiae))
    VPS35 antibody [C3], C-term

    纯化方法

    Purified by antigen-affinity chromatography.

    免疫原

    Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human VPS35. The exact sequence is proprietary.

    亚型

    IgG
  • 应用备注

    Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

    说明

    Positive Control: A549 , H1299 , HCT116 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain , rat brain

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.

    储存液

    ProClin

    注意事项

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Lenoir, Ustunel, Rajesh, Kaur, Moreau, Gruenberg, Overduin: "Phosphorylation of conserved phosphoinositide binding pocket regulates sorting nexin membrane targeting." in: Nature communications, Vol. 9, Issue 1, pp. 993, (2018) (PubMed).

  • 抗原

    VPS35 (Vacuolar Protein Sorting 35 (VPS35))

    别名

    VPS35

    背景

    This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex.

    Cellular Localization: Cytoplasm , Membrane, Peripheral membrane protein

    分子量

    92 kDa

    基因ID

    55737
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