VPS35 抗体 (C-Term)
Quick Overview for VPS35 抗体 (C-Term) (ABIN2856562)
抗原
See all VPS35 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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交叉反应
- 斑马鱼, 小鸡, 牛
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交叉反应 (详细)
- Zebrafish (86 %), Chicken (93 %), Bovine (100 %)
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产品特性
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Rabbit Polyclonal antibody to VPS35 (vacuolar protein sorting 35 homolog (S. cerevisiae))
VPS35 antibody [C3], C-term -
纯化方法
- Purified by antigen-affinity chromatography.
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免疫原
- Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human VPS35. The exact sequence is proprietary.
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亚型
- IgG
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应用备注
- Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
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说明
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Positive Control: A549 , H1299 , HCT116 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain , rat brain
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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Phosphorylation of conserved phosphoinositide binding pocket regulates sorting nexin membrane targeting." in: Nature communications, Vol. 9, Issue 1, pp. 993, (2018) (PubMed).
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Phosphorylation of conserved phosphoinositide binding pocket regulates sorting nexin membrane targeting." in: Nature communications, Vol. 9, Issue 1, pp. 993, (2018) (PubMed).
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- VPS35 (Vacuolar Protein Sorting 35 (VPS35))
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别名
- VPS35
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背景
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This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex.
Cellular Localization: Cytoplasm , Membrane, Peripheral membrane protein -
分子量
- 92 kDa
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基因ID
- 55737
抗原
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