IDH1 抗体 (Center)
Quick Overview for IDH1 抗体 (Center) (ABIN2856056)
抗原
See all IDH1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Center
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交叉反应
- 猕猴
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交叉反应 （详细）
- Rhesus Monkey (100 %)
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产品特性
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Rabbit Polyclonal antibody to IDH1 (isocitrate dehydrogenase 1 (NADP+), soluble)
IDH1 antibody -
纯化方法
- Purified by antigen-affinity chromatography.
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免疫原
- Recombinant protein encompassing a sequence within the center region of human IDH1. The exact sequence is proprietary.
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亚型
- IgG
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anti-Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (AA 1-414) antibody
IDH1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (AA 74-333) antibodyIDH1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (AA 156-298) antibodyIDH1 适用: 人, 小鼠, 猴 WB, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 7G8A1 unconjugated
anti-Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (N-Term) antibodyIDH1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (AA 156-298) antibodyIDH1 适用: 人, 小鼠, 猴 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 4A4A8 unconjugated
anti-Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (AA 381-413), (C-Term) antibodyIDH1 适用: 人, 小鼠, 大鼠 WB, IHC, FACS, IF, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (AA 116-143) antibodyIDH1 适用: 人, 小鼠 WB, FACS, IF, IHC (p) 宿主: 兔 Polyclonal RB18238 unconjugated
anti-Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (AA 63-90) antibodyIDH1 适用: 人, 小鼠 WB, IHC, ELISA, FACS, IF 宿主: 兔 Polyclonal unconjugated
anti-Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (AA 116-143) antibodyIDH1 适用: 人, 小鼠 WB, IHC, ELISA, FACS, IF 宿主: 兔 Polyclonal unconjugated
anti-Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (AA 1-240) antibodyIDH1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
- Suggested dilution Reference IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceIHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:1000* Western blot1:500-1:3000*
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说明
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Positive Control: A431 , HepG2 , JC , C2C12 , 293T , Hela , Neuro2A , GL261 , NIH-3T3 , BCL-1
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))
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别名
- IDH1
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背景
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Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.
Cellular Localization: Cytoplasm , Peroxisome -
分子量
- 47 kDa
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基因ID
- 3417
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途径
- Warburg Effect
抗原
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