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NFE2 抗体 (Center)

This anti-NFE2 antibody is a 兔 多克隆 antibody detecting NFE2 in WB, IF, IP, ICC 和 ChIP-seq. Suitable for 人 和 小鼠.
产品编号 ABIN2855460
发货至: 中国

Quick Overview for NFE2 抗体 (Center) (ABIN2855460)

抗原

See all NFE2 抗体
NFE2 (Nuclear Factor (Erythroid-Derived 2), 45kDa (NFE2))

适用

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人, 小鼠

宿主

  • 35
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克隆类型

  • 33
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多克隆

标记

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This NFE2 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), ChIP DNA-Sequencing (ChIP-seq)
  • 抗原表位

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    Center

    交叉反应

    猴, 猪

    交叉反应 (详细)

    Monkey (97 %), Pig (91 %)

    产品特性

    Rabbit Polyclonal antibody to NF-E2 (nuclear factor (erythroid-derived 2), 45 kDa)
    NF-E2 antibody [N1C3]

    纯化方法

    Purified by antigen-affinity chromatography.

    免疫原

    Recombinant protein encompassing a sequence within the center region of human NF-E2. The exact sequence is proprietary.

    亚型

    IgG
  • 应用备注

    Suggested dilution Reference ICC/IF 1:100-1:1000* Immunoprecipitation Assay-dependent dilution Western blot 1:500-1:3000* ChIP-sequencing Assay-dependent dilution Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* ImmunoprecipitationAssay-dependent dilution Western blot1:500-1:3000* ChIP-sequencingAssay-dependent dilution

    说明

    Positive Control: 293T , mouse spleen

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1.03 mg/mL

    缓冲液

    0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

    储存液

    Thimerosal (Merthiolate)

    注意事项

    This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • 抗原

    NFE2 (Nuclear Factor (Erythroid-Derived 2), 45kDa (NFE2))

    别名

    NF-E2

    背景

    Component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron.

    Cellular Localization: Nucleus

    分子量

    41 kDa

    基因ID

    4778
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