PUF60 抗体 (C-Term)
Quick Overview for PUF60 抗体 (C-Term) (ABIN2855271)
抗原
See all PUF60 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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交叉反应
- 人, 斑马鱼
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产品特性
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Rabbit Polyclonal antibody to PUF60 (poly-U binding splicing factor 60KDa)
PUF60 antibody -
纯化方法
- Purified by antigen-affinity chromatography.
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免疫原
- Recombinant protein encompassing a sequence within the C-terminus region of human PUF60. The exact sequence is proprietary.
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亚型
- IgG
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应用备注
- WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
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说明
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Positive Control: HepG2 , Molt-4 , Raji
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.81 mg/mL
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缓冲液
- 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal
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储存液
- Thimerosal (Merthiolate)
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注意事项
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- PUF60 (Poly-U Binding Splicing Factor 60KDa (PUF60))
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别名
- poly(U) binding splicing factor 60
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背景
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The protein encoded by this gene is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. There are two alternatively spliced transcript variants of this gene encoding different isoforms. There seems to be evidence of multiple polyadenylation sites for this gene.
Cellular Localization: Nucleus -
分子量
- 60 kDa
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基因ID
- 22827
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UniProt
- Q9UHX1
抗原
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