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MIA3 抗体 (N-Term)

The 兔 多克隆 anti-MIA3 antibody is suitable to detect MIA3 in samples from 人. It has been validated for WB, IHC (p), ICC 和 IF.
产品编号 ABIN2855189
发货至: 中国
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中国
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Beijing Economic Technological Development Area
Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for MIA3 抗体 (N-Term) (ABIN2855189)

抗原

See all MIA3 抗体
MIA3 (Melanoma Inhibitory Activity Family, Member 3 (MIA3))

适用

  • 20
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  • 13

宿主

  • 28

克隆类型

  • 28
多克隆

标记

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This MIA3 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (IF)
  • 抗原表位

    • 15
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    N-Term

    交叉反应

    交叉反应 (详细)

    Bovine (89 %)

    产品特性

    Rabbit Polyclonal antibody to MIA3 (melanoma inhibitory activity family, member 3)
    MIA3 antibody [N1], N-term

    纯化方法

    Purified by antigen-affinity chromatography.

    免疫原

    Recombinant protein encompassing a sequence within the N-terminus region of human MIA3. The exact sequence is proprietary.

    亚型

    IgG
  • 应用备注

    Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 10 μg/mL* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)10 μg/mL* Western blot1:500-1:3000*

    说明

    Positive Control: A549 , HepG2

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.30 mg/mL

    缓冲液

    1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

    储存液

    Thimerosal (Merthiolate)

    注意事项

    This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • 抗原

    MIA3 (Melanoma Inhibitory Activity Family, Member 3 (MIA3))

    别名

    MIA3

    背景

    Required for collagen VII (COL7A1) secretion by loading COL7A1 into transport carriers. May participate in cargo loading of COL7A1 at endoplasmic reticulum exit sites by binding to COPII coat subunits Sec23/24 and guiding SH3-bound COL7A1 into a growing carrier. Does not play a role in global protein secretion and is apparently specific to COL7A1 cargo loading. However, it may participate in secretion of other proteins in cells that do not secrete COL7A1.

    Cellular Localization: Endoplasmic reticulum membrane, Single-pass type I membrane protein

    分子量

    214 kDa

    基因ID

    375056
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