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Actin, gamma 1 抗体 (N-Term)

Name: Actin, gamma 1 抗体 (N-Term)
Brand: antibodies-online
SKU: ABIN2855174
Availability: OutOfStock

ACTG1 适用: 人 WB, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

WB Image Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Sarcomeric Actin antibody , diluted at 1:1000. (Actin, gamma 1 抗体 (N-Term))

WB Image Sample (30 ug of whole cell lysate) A: A431 , 10% SDS PAGE antibody diluted at 1:1000 (Actin, gamma 1 抗体 (N-Term))

IHC-P Image Immunohistochemical analysis of paraffin-embedded human Spleen, using gamma Actin, antibody(10 μg/ml). (Actin, gamma 1 抗体 (N-Term))

IHC

WB Image Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 10% SDS PAGE antibody diluted at 1:1000 (Actin, gamma 1 抗体 (N-Term))

Protein expression levels in nondiabetic and diabetic gastric fundus and antrum smooth muscles. (Actin, gamma 1 抗体 (N-Term))

BEZ235 maintenance treatment following RT + BEZ235 treatment resulted in significantly less cell survival and more apoptosis markers in CRC cells. (Actin, gamma 1 抗体 (N-Term))

BEZ235 maintenance treatment following RT + BEZ235 treatment sensitized CRC cells to radiation by attenuating mTOR signaling- and angiogenesis-associated molecules. (Actin, gamma 1 抗体 (N-Term))

8 images

(3 references)

产品编号 ABIN2855174

发货至: 中国

Datasheet as PDF

抗原 See all Actin, gamma 1 (ACTG1) 抗体

Actin, gamma 1 (ACTG1)
抗原表位
5

3

3

3

1

1

1

1

1
N-Term
适用
30

15

12

4

3

3

2

1

1

1

1

1
人
宿主
27

5
兔
Compatible Secondaries
克隆类型
27

5
多克隆
标记
19

4

2

1

1

1

1

1

1

1
This Actin, gamma 1 antibody is un-conjugated
应用范围
20

13

8

8

6

5

4

3

2

2

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

交叉反应

Cat, 犬, 人, 小鼠, 大鼠

产品特性

Rabbit Polyclonal antibody to alpha Sarcomeric Actin (actin, cytoplasmic 2)
alpha Sarcomeric Actin antibody

纯化方法

Purified by antigen-affinity chromatography.

免疫原

Recombinant protein encompassing a sequence within the N-terminus region of human Actin Gamma 1. The exact sequence is proprietary.

亚型

IgG
anti-Actin, gamma 1 (ACTG1) (AA 1-375) antibody
ACTG1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Actin, gamma 1 (ACTG1) (AA 188-215) antibody
ACTG1 适用: 人 WB, ELISA, FACS, IF 宿主: 兔 Polyclonal unconjugated

anti-Actin, gamma 1 (ACTG1) (AA 1-119) antibody
ACTG1 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Actin, gamma 1 (ACTG1) (AA 13-166) antibody
ACTG1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Actin, gamma 1 (ACTG1) (AA 1-375) antibody
ACTG1 适用: 人 ELISA, PLA 宿主: 小鼠 Monoclonal 1G6 unconjugated

anti-Actin, gamma 1 (ACTG1) (AA 188-215) antibody
ACTG1 适用: 人 WB, FACS, IF 宿主: 兔 Polyclonal RB19691 unconjugated

anti-Actin, gamma 1 (ACTG1) (N-Term) antibody
ACTG1 适用: 人 WB, ELISA, ICC, FACS, IHC (p), IHC (fro) 宿主: 小鼠 Monoclonal 2A3 unconjugated

anti-Actin, gamma 1 (ACTG1) (N-Term) antibody
ACTG1 适用: 人, 小鼠 WB, IHC, IHC (p), IF 宿主: 小鼠 Monoclonal 2A3 unconjugated

anti-Actin, gamma 1 (ACTG1) antibody
ACTG1 适用: 人 WB, IF/ICC, FACS 宿主: 兔 Polyclonal unconjugated

anti-Actin, gamma 1 (ACTG1) antibody
ACTG1 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated

应用备注

WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

说明

Positive Control: NIH-3T3 , JC , BCL-1 , A431 , PC-12 , Rat2

限制

仅限研究用
状态

Liquid

浓度

0.2 mg/mL

缓冲液

1XPBS ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

储存液

Thimerosal (Merthiolate)

注意事项

This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Xie, Perrino: "Quantitative in situ proximity ligation assays examining protein interactions and phosphorylation during smooth muscle contractions." in: Analytical biochemistry, Vol. 577, pp. 1-13, (2020) (PubMed).

Chen, Wang, Wei, Tzeng, Lan, Cheng, Kuo: "Maintenance BEZ235 Treatment Prolongs the Therapeutic Effect of the Combination of BEZ235 and Radiotherapy for Colorectal Cancer." in: Cancers, Vol. 11, Issue 8, (2019) (PubMed).

Li, Sasse, Bayguinov, Ward, Perrino: "Contractile Protein Expression and Phosphorylation and Contractility of Gastric Smooth Muscles from Obese Patients and Patients with Obesity and Diabetes." in: Journal of diabetes research, Vol. 2018, pp. 8743874, (2018) (PubMed).
抗原

Actin, gamma 1 (ACTG1)

别名

actin gamma 1 (ACTG1 产品)

别名

ACT antibody, ACTG antibody, BRWS2 antibody, DFNA20 antibody, DFNA26 antibody, ACTG1 antibody, ACTA1 antibody, actg1a antibody, Actg antibody, actg1b antibody, AL023024 antibody, Actl antibody, E51 antibody, actin antibody, cytoplasmic antibody, actin gamma 1 antibody, actin, gamma 1 a antibody, actin, gamma 1 antibody, actin gamma 1 S homeolog antibody, actin, gamma, cytoplasmic 1 antibody, Actin, gamma 1 antibody, ACTG1 antibody, actg1 antibody, Actg1 antibody, actg1.S antibody

背景

Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in nonmuscle cells.

Cellular Localization: Cytoplasm , cytoskeleton

分子量

42 kDa

基因ID

71

UniProt

P63261

途径

Myometrial Relaxation and Contraction, Cell-Cell Junction Organization