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Actin, gamma 1 抗体 (N-Term)

This anti-Actin, gamma 1 antibody is a 兔 多克隆 antibody detecting Actin, gamma 1 in WB, IHC (p) 和 IHC (fro). Suitable for 人. This Primary Antibody has been cited in 3+ publications.
产品编号 ABIN2855174
发货至: 中国

Quick Overview for Actin, gamma 1 抗体 (N-Term) (ABIN2855174)

抗原

See all Actin, gamma 1 (ACTG1) 抗体
Actin, gamma 1 (ACTG1)

适用

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宿主

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克隆类型

  • 35
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多克隆

标记

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This Actin, gamma 1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • 抗原表位

    • 6
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    • 1
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    N-Term

    交叉反应

    Cat, 犬, 人, 小鼠, 大鼠

    产品特性

    Rabbit Polyclonal antibody to alpha Sarcomeric Actin (actin, cytoplasmic 2)
    alpha Sarcomeric Actin antibody

    纯化方法

    Purified by antigen-affinity chromatography.

    免疫原

    Recombinant protein encompassing a sequence within the N-terminus region of human Actin Gamma 1. The exact sequence is proprietary.

    亚型

    IgG
  • 应用备注

    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

    说明

    Positive Control: NIH-3T3 , JC , BCL-1 , A431 , PC-12 , Rat2

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.2 mg/mL

    缓冲液

    1XPBS ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

    储存液

    Thimerosal (Merthiolate)

    注意事项

    This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Xie, Perrino: "Quantitative in situ proximity ligation assays examining protein interactions and phosphorylation during smooth muscle contractions." in: Analytical biochemistry, Vol. 577, pp. 1-13, (2020) (PubMed).

    Chen, Wang, Wei, Tzeng, Lan, Cheng, Kuo: "Maintenance BEZ235 Treatment Prolongs the Therapeutic Effect of the Combination of BEZ235 and Radiotherapy for Colorectal Cancer." in: Cancers, Vol. 11, Issue 8, (2019) (PubMed).

    Li, Sasse, Bayguinov, Ward, Perrino: "Contractile Protein Expression and Phosphorylation and Contractility of Gastric Smooth Muscles from Obese Patients and Patients with Obesity and Diabetes." in: Journal of diabetes research, Vol. 2018, pp. 8743874, (2018) (PubMed).

  • 抗原

    Actin, gamma 1 (ACTG1)

    别名

    actin gamma 1

    背景

    Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in nonmuscle cells.

    Cellular Localization: Cytoplasm , cytoskeleton

    分子量

    42 kDa

    基因ID

    71

    UniProt

    P63261

    途径

    Myometrial Relaxation and Contraction, Cell-Cell Junction Organization
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