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ADAR 抗体

This anti-ADAR antibody is a 兔 多克隆 antibody detecting ADAR in WB, IF, IP, IHC (p) 和 ICC. Suitable for 人. This Primary Antibody has been cited in 1 publication.
产品编号 ABIN2855100
发货至: 中国

Quick Overview for ADAR 抗体 (ABIN2855100)

抗原

See all ADAR 抗体
ADAR (Adenosine Deaminase, RNA-Specific (ADAR))

适用

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宿主

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克隆类型

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多克隆

标记

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This ADAR antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

质量等级

KO Validated
  • 交叉反应

    人, 大鼠

    产品特性

    Rabbit Polyclonal antibody to ADAR1 (adenosine deaminase, RNA-specific)
    ADAR1 antibody [N3C1], Internal

    纯化方法

    Purified by antigen-affinity chromatography.

    免疫原

    Recombinant protein encompassing a sequence within the center region of human ADAR1. The exact sequence is proprietary.

    亚型

    IgG
  • 应用备注

    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

    说明

    Positive Control: 293T , A431 , HeLa , HepG2

    Validation: KO/KD

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.82 mg/mL

    缓冲液

    1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

    储存液

    ProClin

    注意事项

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Stejskal, Rájecká, Covelo-Molares, Sinigaglia, Brožinová, Kašiarová, Dohnálková, Reyes-Gutierrez, Cahová, Keegan, O'Connell, Vaňáčová: "Global analysis by LC-MS/MS of N6-methyladenosine and inosine in mRNA reveal complex incidence." in: RNA (New York, N.Y.), Vol. 31, Issue 4, pp. 514-528, (2025) (PubMed).

  • 抗原

    ADAR (Adenosine Deaminase, RNA-Specific (ADAR))

    别名

    adenosine deaminase RNA specific

    背景

    This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

    Cellular Localization: Cytoplasm , Nucleus , nucleolus

    分子量

    136 kDa

    基因ID

    103

    UniProt

    P55265

    途径

    Protein targeting to Nucleus
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