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COX IV 抗体 (Center)

Name: COX IV 抗体 (Center)
Brand: antibodies-online
SKU: ABIN2855069
Availability: OutOfStock

COX4I1 适用: 人 WB, IF, ICC, IHC (p), IP 宿主: 兔 Polyclonal unconjugated

WB Image Sample(30 μg of whole cell lysate) A:A431, B:MOLT4, 12% SDS PAGE antibody diluted at 1:2000 (COX IV 抗体 (Center))

ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using COX4, antibody (Green) at 1:500 dilution. (COX IV 抗体 (Center))

IP Image Immunoprecipitation of COX4 protein from 293T whole cell extracts using 5 μg of COX4 antibody, Western blot analysis was performed using COX4 antibody, EasyBlot anti-Rabbit IgG was used as a secondary reagent. (COX IV 抗体 (Center))

IHC-P Image Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using COX4, antibody at 1:100 dilution. (COX IV 抗体 (Center))

IHC

NAC/AAP decreased the activation of Drp1 inH2O2-treated hADMSCs. (COX IV 抗体 (Center))

Spermidine induced ATM-dependent formation of mitophagosomes and mitolysosomes and decreased mitochondrial mass. (COX IV 抗体 (Center))

Spermidine affected the accumulation of PINK1 and translocation of Parkin. (COX IV 抗体 (Center))

IF (cc)

8 images

产品编号 ABIN2855069

发货至: 中国

Datasheet as PDF

抗原 See all COX IV (COX4I1) 抗体

COX IV (COX4I1) (Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1))
抗原表位
16

15

11

8

8

8

6

3

3

2

2

1

1

1

1

1

1

1

1

1
Center
适用
119

64

53

4

4

3

2

2

1

1

1

1

1
人
宿主
84

39

2

2
兔
Compatible Secondaries
克隆类型
79

48
多克隆
标记
75

6

5

4

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1
This COX IV antibody is un-conjugated
应用范围
108

45

37

32

27

20

20

18

16

14

6

3

3

2

1
Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

交叉反应

Chimpanzee

交叉反应（详细）

Chimpanzee (100 %)

产品特性

Rabbit Polyclonal antibody to COX4 (cytochrome c oxidase subunit IV isoform 1)
COX4 antibody

纯化方法

Purified by antigen-affinity chromatography.

免疫原

Recombinant protein encompassing a sequence within the center region of human COX4. The exact sequence is proprietary.

亚型

IgG

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anti-Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1) (N-Term) antibody
COX4I1 适用: 人, 大鼠, 小鼠, Cow, 犬, 豚鼠, 马, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1) (AA 59-169) antibody
COX4I1 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated

anti-Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1) (AA 51-169) antibody
COX4I1 适用: 人, 大鼠, 小鼠 WB, ELISA, FACS, ICC, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1) (AA 1-169) antibody
COX4I1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 4A10 unconjugated

anti-Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1) (AA 23-169) antibody
COX4I1 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1) antibody
COX4I1 适用: 人, 大鼠, 小鼠, 猴 WB, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 6B3 unconjugated

anti-Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1) antibody
COX4I1 适用: 人, 大鼠 WB, IHC (p), IF (p) 宿主: 小鼠 Monoclonal 8D8 unconjugated

anti-Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1) antibody
COX4I1 适用: 人, 大鼠, 小鼠 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 2D4 unconjugated

anti-Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1) (N-Term) antibody
COX4I1 适用: 人, 小鼠, 犬 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

应用备注

Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*

说明

Positive Control: 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

限制

仅限研究用
状态

Liquid

浓度

0.38 mg/mL

缓冲液

0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

储存液

Thimerosal (Merthiolate)

注意事项

This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
抗原

COX IV (COX4I1) (Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1))

别名

COX4 (COX4I1 产品)

背景

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it.

Cellular Localization: Mitochondrion inner membrane

分子量

20 kDa

基因ID

1327

途径

Proton Transport