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OCT4 抗体

Name: OCT4 抗体
Brand: antibodies-online
SKU: ABIN2855068
Availability: OutOfStock

This anti-OCT4 antibody is a 兔多克隆 antibody detecting OCT4 in WB, IF, ICC, IP, FACS, IHC (p) 和 IHC (fro). Suitable for 人. This Primary Antibody has been cited in 4+ publications.

WB Image OCT4 antibody detects OCT4 protein by western blot analysis. (OCT4 抗体)

FACS

ICC/IF Image Immunofluorescence analysis of hESCs on CF1 feeders, using OCT3/4, antibody at 1:200 dilution. (OCT4 抗体)

IP Image Oct4 antibody immunoprecipitates Oct4 protein in IP experiments. (OCT4 抗体)

WB Image Sample (20 ug of whole cell lysate) A: mouse ESC 12% SDS PAGE antibody diluted at 1:10000 (OCT4 抗体)

ICC/IF Image Immunofluorescence analysis of mESCs on CF1 feeders, using OCT3/4, antibody at 1:200 dilution. (OCT4 抗体)

IHC-P Image Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using OCT3/4, antibody at 1:500 dilution. (OCT4 抗体)

IHC

8 images

(4 references)

产品编号 ABIN2855068

发货至: 中国

Quick Overview for OCT4 抗体 (ABIN2855068)

抗原

See all OCT4 (POU5F1) 抗体

OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

适用

人

宿主

兔

克隆类型

多克隆

标记

This OCT4 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

质量等级

KO Validated

Discover our top product POU5F1 Primary Antibody

交叉反应

人, 小鼠, 大鼠

产品特性

Rabbit Polyclonal antibody to OCT4 (POU class 5 homeobox 1)
Oct4 antibody

纯化方法

Purified by antigen-affinity chromatography.

免疫原

Recombinant protein encompassing a sequence within the center region of human Oct4. The exact sequence is proprietary.

亚型

IgG
anti-POU Class 5 Homeobox 1 (POU5F1) (AA 81-164) antibody
POU5F1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1B11 unconjugated

anti-POU Class 5 Homeobox 1 (POU5F1) (AA 201-300) antibody
POU5F1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-POU Class 5 Homeobox 1 (POU5F1) (AA 1-360) antibody
POU5F1 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 小鼠 Monoclonal 2H41D8 unconjugated

anti-POU Class 5 Homeobox 1 (POU5F1) antibody
POU5F1 适用: 人 WB, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 7E7 unconjugated

anti-POU Class 5 Homeobox 1 (POU5F1) (AA 136-360) antibody
POU5F1 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-POU Class 5 Homeobox 1 (POU5F1) (AA 1-360) antibody
POU5F1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-POU Class 5 Homeobox 1 (POU5F1) (AA 81-164) antibody
POU5F1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1D2 unconjugated

anti-POU Class 5 Homeobox 1 (POU5F1) (Internal Region) antibody
POU5F1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-POU Class 5 Homeobox 1 (POU5F1) antibody
POU5F1 适用: 人, 猴 WB, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-POU Class 5 Homeobox 1 (POU5F1) (AA 86-119), (N-Term) antibody
POU5F1 适用: 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB52378 unconjugated

应用备注

WB: 1:5000-1:100000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. FACS: 1:50-1:200. IP: 1:500-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

说明

Positive Control: mouse ESC , human ESC , NT2D1
Validation: KO/KD, Orthogonal, Overexpression

限制

仅限研究用
状态

Liquid

浓度

0.49 mg/mL

缓冲液

1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Feng, Su, Lin, Lin, Wu, Tung, Li, Shieh, Shiau: "Oct4 upregulates osteopontin via Egr1 and is associated with poor outcome in human lung cancer." in: BMC cancer, Vol. 19, Issue 1, pp. 791, (2020) (PubMed).

Lee, Liu, Chang, Nieh, Lin, Jao, Chen, Liu: "Increased chemoresistance via Snail-Raf kinase inhibitor protein signaling in colorectal cancer in response to a nicotine derivative." in: Oncotarget, Vol. 7, Issue 17, pp. 23512-20, (2018) (PubMed).

Ju, Huang, Huang, Su: "Identification of thiostrepton as a novel therapeutic agent that targets human colon cancer stem cells." in: Cell death & disease, Vol. 6, pp. e1801, (2016) (PubMed).

Chen, Huang, Lin: "Sphere-forming-like cells (squamospheres) with cancer stem-like cell traits from VX2 rabbit buccal squamous cell carcinoma." in: International journal of oral science, Vol. 6, Issue 4, pp. 212-8, (2015) (PubMed).
抗原

OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

别名

POU class 5 homeobox 1

背景

This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6,22)(p21,q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.

Cellular Localization: Nucleus

分子量

39 kDa

基因ID

5460

UniProt

Q01860

途径

Stem Cell Maintenance