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ALDOB 抗体

This anti-ALDOB antibody is a 兔 多克隆 antibody detecting ALDOB in WB, IF, IHC (p) 和 ICC. Suitable for 人. This Primary Antibody has been cited in 2+ publications.
产品编号 ABIN2855024
发货至: 中国

Quick Overview for ALDOB 抗体 (ABIN2855024)

抗原

See all ALDOB 抗体
ALDOB (Aldolase B, Fructose-Bisphosphate (ALDOB))

适用

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宿主

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克隆类型

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多克隆

标记

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This ALDOB antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
  • 交叉反应

    人, 小鼠, 大鼠, Turtle

    产品特性

    Rabbit Polyclonal antibody to Aldolase B (aldolase B, fructose-bisphosphate)
    Aldolase B antibody

    纯化方法

    Purified by antigen-affinity chromatography.

    免疫原

    Recombinant protein encompassing a sequence within the center region of human Aldolase B. The exact sequence is proprietary.

    亚型

    IgG
  • 应用备注

    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

    说明

    Positive Control: Neuro2A , GL261 , C8D30 , PC-12 , mouse brain , rat brain , 293T , A431 , HeLa , HepG2

    Validation: Orthogonal

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.09 mg/mL

    缓冲液

    1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

    储存液

    ProClin

    注意事项

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Chen, Lin, Chen, Cheng, Cheng, Imai et al.: "Effect of prednisolone on glyoxalase 1 in an inbred mouse model of aristolochic acid nephropathy using a proteomics method with fluorogenic derivatization-liquid chromatography-tandem mass ..." in: PLoS ONE, Vol. 15, Issue 1, pp. e0227838, (2020) (PubMed).

    Dawson, Biggar, Storey: "Characterization of fructose-1,6-bisphosphate aldolase during anoxia in the tolerant turtle, Trachemys scripta elegans: an assessment of enzyme activity, expression and structure." in: PLoS ONE, Vol. 8, Issue 7, pp. e68830, (2014) (PubMed).

  • 抗原

    ALDOB (Aldolase B, Fructose-Bisphosphate (ALDOB))

    别名

    aldolase, fructose-bisphosphate B

    背景

    Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5 % of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance.

    分子量

    39 kDa

    基因ID

    229

    UniProt

    P05062
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