HPRT1 抗体
Quick Overview for HPRT1 抗体 (ABIN2854953)
抗原
See all HPRT1 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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交叉反应
- 人, 大鼠, 斑马鱼
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产品特性
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Rabbit Polyclonal antibody to HPRT (hypoxanthine phosphoribosyltransferase 1)
HPRT antibody -
纯化方法
- Purified by antigen-affinity chromatography.
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免疫原
- Recombinant protein encompassing a sequence within the center region of human HPRT. The exact sequence is proprietary.
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亚型
- IgG
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应用备注
- WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
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说明
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Positive Control: PC-12 , Rat2 , 293T , A431 , HeLa , HepG2 , rat brain
Validation: KO/KD
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal
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储存液
- Thimerosal (Merthiolate)
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注意事项
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- HPRT1 (Hypoxanthine phosphoribosyltransferase 1 (HPRT1))
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别名
- hypoxanthine phosphoribosyltransferase 1
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背景
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HPRT1 has a central role in the generation of purine nucleotides through the purine salvage pathway. HPRT1 catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate (Keebaugh et al., 2007 [PubMed 16928426]).[supplied by OMIM]
Cellular Localization: Cytoplasm -
分子量
- 25 kDa
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基因ID
- 3251
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UniProt
- P00492
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途径
- Ribonucleoside Biosynthetic Process
抗原
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