IL3RA 抗体 (FITC)
Quick Overview for IL3RA 抗体 (FITC) (ABIN2853580)
抗原
See all IL3RA 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody reacts with Human CD123 antigen (IL-3 receptor alpha-chain).
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交叉反应 （详细）
- Species reactivity (tested):Human.
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纯化方法
- Protein-A Sepharose Chromatography.
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免疫原
- COS cells overexpressing IL-3 receptor alpha-chain. Remarks: Hybridoma was established by fusion of mouse myeloma cell NS-1 with Balb/c mouse splenocyte
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亚型
- IgG1
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anti-Interleukin 3 Receptor, alpha (IL3RA) (AA 200-305) antibody
IL3RA 适用: 人 FACS, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 8E11C5 unconjugated
anti-Interleukin 3 Receptor, alpha (IL3RA) (AA 26-171) antibodyIL3RA 适用: 人 IHC, ISt, StM 宿主: 小鼠 Monoclonal IL3RA-1531 unconjugated
anti-Interleukin 3 Receptor, alpha (IL3RA) antibody (PE)IL3RA 适用: 人 FACS 宿主: 小鼠 Monoclonal 6H6 PE
anti-Interleukin 3 Receptor, alpha (IL3RA) (AA 19-158) antibodyIL3RA 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 3 Receptor, alpha (IL3RA) (AA 201-300) antibodyIL3RA 适用: 人, 小鼠 FACS, WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 3 Receptor, alpha (IL3RA) (AA 167-331) antibodyIL3RA 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 3 Receptor, alpha (IL3RA) antibodyIL3RA 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 3 Receptor, alpha (IL3RA) (AA 26-171) antibodyIL3RA 适用: 人 IHC, ELISA, ISt, StM, Coat 宿主: 兔 Monoclonal IL3RA-2947R unconjugated Recombinant Antibody
anti-Interleukin 3 Receptor, alpha (IL3RA) antibodyIL3RA 适用: 人 FACS, WB, IP, IHC (fro) 宿主: 小鼠 Monoclonal 6H6 unconjugated
anti-Interleukin 3 Receptor, alpha (IL3RA) (AA 19-305) antibodyIL3RA 适用: 人 FACS, ELISA 宿主: 兔 Monoclonal DM31 unconjugated
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应用备注
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Flow Cytometry: 10-20 μg/mL (final concentration). Positive Control: Transfectant cells. Detailed procedure is provided in Protocols.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- Flow Cytometric Aanalysis for Floating CellsProtocol 1We use Fisher tubes or equivalents as reaction tubes for all steps described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and0. 1% NaN3]. 2) Add 10 μL of normal goat serum to the cell pellet after tapping. Mix well, and incubatefor 5 minutes at room temperature (20~25°C). 3) Add 30 µL of the FITC labeled CD123 monoclonal antibody (10-20 μg/mL) diluted with thewashing buffer. Mix well, and incubate for 30 minutes at room temperature (20~25°C). 4) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at roomtemperature (20~25°C). Remove supernatant by careful aspiration. 5) Resuspend the cells with 500 μL of the washing buffer and analyze by a flow cytometer. Protocol 2We usually use Fisher tubes or equivalents as reaction tubes for all steps described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and0. 1% NaN3]. 2) Resuspend the cells with PBS containing 25% normal goat serum and 0. 1% NaN3(5x10e6 cells/mL). 3) Add 20 µL of the non diluted FITC labeled CD123 monoclonal antibody into each tube. 4) Add 50 µL of the cell suspension into each tube. Mix well, and incubate for 30 minutesat room temperature (20~25°C). 5) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at roomtemperature (20~25°C). Remove supernatant by careful aspiration. 6) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer. Flow Cytometric analysis for whole blood cellsWe usually use Falcon tubes or equivalents as reaction tubes for all steps described below. 1) Add 20 μL of the non diluted FITC labeled CD123 monoclonal antibody into each tube. 2) Add 50 μL of whole blood into each tube. Mix well, and incubate for 30 minutes at roomtemperature (20~25°C). 3) Lyse with OptiLyse C (for analysis on Beckman Coulterinstruments) or OptiLyse B (foranalysis on BD instruments), using the procedure recommended in the respective packageinserts. 4) Resuspend the cells with 500 μL of the washing buffer [PBS containing 2% fetal calfserum (FCS) and 0. 1% NaN3] and analyze by a flow cytometer. Positive Control for Flow Cytometry: Peripheral blood monocytes.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.05 mg/mL
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缓冲液
- PBS containing 1 % BSA as stabilizer and 0.09 % Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store the antibody undiluted at 2-8 °C.
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- IL3RA (Interleukin 3 Receptor, alpha (IL3RA))
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别名
- CD123 / IL3RA
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背景
- CD123 is the IL-3-specific component of the IL-3 receptor. CD123 binds IL-3 only in combination with CDw131, the Synonyms: IL-3R-alpha, IL3 receptor alpha, Interleukin-3 receptor subunit alpha
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基因ID
- 3563
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NCBI登录号
- NP_002174
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UniProt
- P26951
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途径
- JAK/STAT Signaling
抗原
-
