(1 reference)-
- 抗原
- NPT II
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- 非结合性
- 应用范围
- Western Blotting (WB), ELISA
- 免疫原
- MPTII antibody was raised in rabbit using purified neomycin phosphotransferase II as the immunogen.
- 亚型
- IgG
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anti-NPT II antibody
适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4B4D1 unconjugated
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- 应用备注
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Use at starting dilution 1:500
Optimal conditions should be determined by the investigator. - 限制
- 仅限研究用
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- 浓度
- Lot specific
- 缓冲液
- Supplied as an IgG fraction in PBS, pH 7.4 with 0.05 % NaN3
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 注意事项
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Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use. - 储存条件
- 4 °C/-20 °C
- 储存方法
- Store at 4 °C for short term storage. Aliquot and store at -80 °C for long term storage.
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: "Pseudomonas syringae HrpJ is a type III secreted protein that is required for plant pathogenesis, injection of effectors, and secretion of the HrpZ1 Harpin." in: Journal of bacteriology, Vol. 188, Issue 17, pp. 6060-9, (2006) (PubMed).
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: "Pseudomonas syringae HrpJ is a type III secreted protein that is required for plant pathogenesis, injection of effectors, and secretion of the HrpZ1 Harpin." in: Journal of bacteriology, Vol. 188, Issue 17, pp. 6060-9, (2006) (PubMed).
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- 抗原
- NPT II
- 别名
- NPTII
- 背景
- Neomycin phosphotransferase II confers resistance to kanamycin and neomycin in bacteria and G418 in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell. Immunoblot analysis of transfected cell lysates with anti NPT II can be used to gauge relative transfection efficiencies in experiments where multiple transfections are performed in parallel with different plasmids.
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