-
- 抗原
- SNAI
- 适用
- 人
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- 非结合性
- 应用范围
- Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 纯化方法
- This antibody is purified through a protein A column, followed by peptide affinity purification.
- 免疫原
- This SNAI antibody is generated from rabbits immunized with SNAI recombinant protein.
- 克隆位点
- RB43104
- 亚型
- Ig Fraction
-
anti-SNAI antibody
适用: 人 WB, IHC 宿主: 小鼠 Monoclonal unconjugated
-
- 应用备注
-
Suggested dilutions:
WB = 1:1000, IHC (p) = 1:10-50, FACS = 1:10-50
Optimal working dilution should be determined by the investigator. - 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 0.38 mg/mL
- 缓冲液
- PBS with 0.09 % (W/V) sodium azide
- 储存液
- Sodium azide
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 注意事项
- Avoid freeze-thaw cycles.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
- 有效期
- 6 months
-
- 抗原
- SNAI
- 背景
-
Function
Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. Associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up- regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3. In addition, may also activate the CDKN2B promoter by itself.
Cellular Location
Nucleus. Cytoplasm. Note=Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs
Tissue Location
Expressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines
Synonyms: Zinc finger protein SNAI1, Protein snail homolog 1, Protein sna, SNAH, SNAI1
Pathways: Adherens junction - 分子量
- 29083 Da
- 基因ID
- 6615
- UniProt
- O95863
-
You are here:
