GRB10/14 (AA 501-594) 抗体 (AbBy Fluor® 594)
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北京 101111
Quick Overview for GRB10/14 (AA 501-594) 抗体 (AbBy Fluor® 594) (ABIN2814026)
抗原
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 501-594
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交叉反应
- 人, 小鼠
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预测反应
- Rat,Dog,Cow,Pig,Horse,Rabbit
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纯化方法
- Purified by Protein A.
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免疫原
- KLH conjugated synthetic peptide derived from human GRB10
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亚型
- IgG
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anti-GRB10/14 (AA 501-594) antibody
适用: 人, 小鼠 WB, IF (cc), IF (p), ELISA, IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 μg/μL
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缓冲液
- Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
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有效期
- 12 months
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- GRB10/14
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背景
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Synonyms: GRB IR, grb-10, GRB10 adaptor protein, GRBIR, Growth factor receptor bound protein 10, Insulin receptor binding protein, Insulin receptor binding protein GRB IR, IRBP, MEG1, RSS, GRB14, GRB14 adapter protein, Growth factor receptor-bound protein 14.
Background: Adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. Binds to, and suppress signals from, activated receptors tyrosine kinases, including the insulin (INSR) and insulin-like growth factor (IGF1R) receptors. The inhibitory effect can be achieved by 2 mechanisms: interference with the signaling pathway and increased receptor degradation. Delays and reduces AKT1 phosphorylation in response to insulin stimulation. Blocks association between INSR and IRS1 and IRS2 and prevents insulin-stimulated IRS1 and IRS2 tyrosine phosphorylation. Recruits NEDD4 to IGF1R, leading to IGF1R ubiquitination, increased internalization and degradation by both the proteasomal and lysosomal pathways. May play a role in mediating insulin-stimulated ubiquitination of INSR, leading to proteasomal degradation. Negatively regulates Wnt signaling by interacting with LRP6 intracellular portion and interfering with the binding of AXIN1 to LRP6. Positive regulator of the KDR/VEGFR-2 signaling pathway. May inhibit NEDD4-mediated degradation of KDR/VEGFR-2. GRB14 is a potent inhibitor of insulin-stimulated MAPK3 phosphorylation. Plays a critical role regulating PDPK1 membrane translocation in response to insulin stimulation and serves as an adapter protein to recruit PDPK1 to activated insulin receptor, thus promoting PKB/AKT1 phosphorylation and transduction of the insulin signal.
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基因ID
- 2887, 2888
抗原
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