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RAG1 抗体 (AA 351-450) (AbBy Fluor® 594)

This anti-RAG1 antibody is a 兔 多克隆 antibody detecting RAG1 in WB, FACS, IF (cc) 和 IF (p). Suitable for 小鼠.
产品编号 ABIN2806049
发货至: 中国

Quick Overview for RAG1 抗体 (AA 351-450) (AbBy Fluor® 594) (ABIN2806049)

抗原

See all RAG1 抗体
RAG1 (Recombination Activating Gene 1 (RAG1))

适用

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小鼠

宿主

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克隆类型

  • 22
  • 3
多克隆

标记

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This RAG1 antibody is conjugated to AbBy Fluor® 594

应用范围

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Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))
  • 抗原表位

    • 15
    • 4
    • 2
    • 1
    • 1
    AA 351-450

    交叉反应

    小鼠

    预测反应

    Human,Rat,Sheep,Pig

    纯化方法

    Purified by Protein A.

    免疫原

    KLH conjugated synthetic peptide derived from human RAG1/RNF74

    亚型

    IgG
  • 应用备注

    FCM 1:20-100
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 μg/μL

    缓冲液

    Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

    储存液

    ProClin

    注意事项

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

    有效期

    12 months
  • 抗原

    RAG1 (Recombination Activating Gene 1 (RAG1))

    别名

    RNF74

    背景

    Synonyms: RAG-1, RNF74, V(D)J recombination-activating protein 1, RING finger protein 74, RAG1

    Background: Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In addition to its endonuclease activity, RAG1 also acts as a E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3. Histone H3 monoubiquitination is required for the joining step of V(D)J recombination.

    基因ID

    5896

    UniProt

    P15918
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